Preparation And Storage
Recommended Assay Procedures
This antibody is useful for western blotting and for immunofluorescent labelling of cells for fluorescence microscopy or flow cytometry. For flow
cytometric applications, a three-step labelling procedure is recommended for amplifying signal. For example:
1. Incubate (~1 million) cells with ~2 µg of antibody for 45-60 minutes at 4° C. Wash three times in PBS/0.1% sodium azide/1.0% heat -inactivated fetal bovine serum (PBS-FBS).
2. Incubate cells with ~0.5 µg biotinylated goat anti-mouse IgS (Cat. No. 553999) at 4° C for 30 minutes. Wash three times.
3. Incubate cells with ~0.1 µg streptavidin-PE (Cat. No. 554061) at 4° C for 30 minutes. Wash three times. Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
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The 9F5 monoclonal antibody specifically binds to CD123. CD123 is the 70 kDa IL-3 receptor α chain (IL-3Rα) that associates with the 120-140 kDa β subunit (CD131/Common β-chain/βc) to form the functional IL-3 receptor complex. The βc chain is also shared with distinct α chain subunits to form the functional heterodimeric receptors for interleukins IL-5 and GM-CSF. IL-3Rα is expressed on a subset of peripheral blood dendritic cells, myeloid precursors, basophils, mast cells, macrophages, and megakaryocytes. Reports indicate that IL-3Rα is also expressed on lymphocytes. The IL-3R plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody does not block binding of IL-3 to the IL-3 receptor.
Development References (2)
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Macardle PJ, Chen Z, Shih CY, et al. Characterization of human leucocytes bearing the IL-3 receptor. Cell Immunol. 1996; 168(1):59-68. (Biology). View Reference
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Sun Q, Woodcock JM, Rapoport A, et al. Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist.. Blood. 1996; 87(1):83-92. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.