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Purified Mouse Anti-Human CD112
Product Details
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BD Pharmingen™
HVEB; PRR2; PVRL2; PVRR2; NECTIN2; nectin-2; nectin 2
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551056 Rev. 4
Antibody Details
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The R2.525 monoclonal antibody specifically binds to CD112. CD112 is a type I transmembrane glycoprotein that is encoded by NECTIN2 (Nectin cell adhesion molecule 2) which belongs to the Ig gene superfamily. CD112 is also known as polio virus related receptor 2 (PRR2), poliovirus receptor-like 2 (PVRL2) or herpesvirus entry protein B (HveB). CD112 is a cell surface molecule homologous to the poliovirus receptor (PVR/CD155). The NECTIN2 gene codes for two mRNA isoforms, a short form of 3.0 kb (hPRR2α) and a long form of 4.4 kb (hPRR2δ). Both are widely expressed in human tissues, including hematopoietic cells. CD112 is also expressed on endothelial cells, at the intercellular junctions of adjacent cells. PRR2 may play a role in the regulation of hematopoietic/endothelial cell interactions.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

551056 Rev. 4
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
551056 Rev.4
Citations & References
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Development References (2)

  1. Eberle F, Dubreuil P, Mattei MG, Devilard E, Lopez M. The human PRR2 gene, related to the human poliovirus receptor gene (PVR), is the true homolog of the murine MPH gene. Gene. 1995; 159(2):267-272. (Biology). View Reference
  2. Lopez M, Aoubala M, Jordier F, Isnardon D, Gomez S, Dubreuil P. The human poliovirus receptor related 2 protein is a new hematopoietic/endothelial homophilic adhesion molecule. Blood. 1998; 92(12):4602-4611. (Biology). View Reference
551056 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.