PE Rat Anti-Mouse CD192 (CCR2)

BD Pharmingen™ PE Rat Anti-Mouse CD192 (CCR2)

Name: PE Rat Anti-Mouse CD192 (CCR2)
Brand: PE Rat Anti-Mouse CD192 (CCR2)
SKU: 571198

Clone C2Mab-6.rMAb (also known as C2Mab-6) (RUO)

Multicolor flow cytometric analysis of CD192 (CCR2) expression on viable Mouse splenic leukocytes. C57BL/6 (Top Plots) and BALB/c (Bottom Plots) Mouse spleen cells were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141). The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat No. 553129), and with either PE Rat IgG1, k Isotype Control (Cat. No. 553925; Left Plots) or PE Rat Anti-Mouse CD192 (CCR2) antibody (Cat. No. 571198/571199; Right Plots) at 0.5 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD192 (CCR2) [or Ig Isotype control staining] versus Ly-6G and Ly-6C were derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Cc-ckr-2; Ccr2; Ckr2; Cmkbr2; MCP-1 receptor

Reactivity: Mouse (QC Testing)

Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ

Immunogen: Mouse CCR2 Peptide

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 12772

RRID: AB_3686326

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

APC Rat Anti-Mouse Ly-6G and Ly-6C RUO

Size 0.1 mg Cat No. 553129

Lysing Buffer RUO

Size 100 mL Cat No. 555899

571198 Rev. 1

Antibody Details

C2Mab-6.rMAb

C2MAB-6.RMAB is a recombinant monoclonal antibody that specifically recognizes CD192 which is also known as C-C chemokine receptor type 2 (CCR2 or CC-CKR-2), Cmkbr2, or MCP-1 receptor (MCP-1-R). CD192 (CCR2) is an ~42 kDa seven-transmembrane, G-protein-coupled, glycoprotein receptor encoded by Ccr2 which belongs to the beta chemokine receptor family. It is expressed on monocytes, macrophages, basophils and on some T cells and dendritic cells. Chemokines including CCL2 (Monocyte chemoattractant protein 1/MCP-1), CCL7 (MCP-3), or CCL12 (MCP-5) bind to and signal through CD192 (CCR2) to recruit monocytes and other leucocytes into inflammatory sites including tumors.

571198 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

571198 Rev.1

Citations & References

View product citations for antibody "571198" on CiteAb

Development References (6)

Engel DR, Maurer J, Tittel AP, et al. CCR2 mediates homeostatic and inflammatory release of Gr1(high) monocytes from the bone marrow, but is dispensable for bladder infiltration in bacterial urinary tract infection.. J Immunol. 2008; 181(8):5579-86. (Biology). View Reference
Fujimura N, Xu B, Dalman J, Deng H, Aoyama K, Dalman RL. CCR2 inhibition sequesters multiple subsets of leukocytes in the bone marrow.. Sci Rep. 2015; 5:11664. (Biology). View Reference
Geissmann F, Jung S, Littman DR. Blood monocytes consist of two principal subsets with distinct migratory properties.. Immunity. 2003; 19(1):71-82. (Biology). View Reference
Mack M, Cihak J, Simonis C, et al. Expression and characterization of the chemokine receptors CCR2 and CCR5 in mice.. J Immunol. 2001; 166(7):4697-704. (Biology: ELISA). View Reference
Tanaka T, Li G, Asano T, et al. Development of a Novel Anti-Mouse CCR2 Monoclonal Antibody (C2Mab-6) by N-Terminal Peptide Immunization.. Monoclon Antib Immunodiagn Immunother. 2022; 41(2):80-86. (Immunogen: ELISA, Flow cytometry). View Reference
Tanaka T, Suzuki H, Asano T, et al. Epitope Mapping of an Anti-Mouse CCR2 Monoclonal Antibody (C2Mab-6) Using Enzyme-Linked Immunosorbent Assay.. Monoclon Antib Immunodiagn Immunother. 2022; 41(6):339-342. (Clone-specific: ELISA). View Reference

View All (6)

571198 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.