PE Mouse Anti-Human VEGFR2 (CD309)

BD Pharmingen™ PE Mouse Anti-Human VEGFR2 (CD309)

Name: PE Mouse Anti-Human VEGFR2 (CD309)
Brand: PE Mouse Anti-Human VEGFR2 (CD309)
SKU: 571194

Clone BB13-1044 (RUO)

Flow cytometric analysis of Human VEGFR2 (CD309) expression on viable HUVEC cells. Human Umbilical Vein Endothelial Cells (HUVEC; Lonza, Cat. No. C2519A) were grown in EGM®-2 Endothelial Cell Growth Medium (Lonza, Cat No. CC-3162), which contains Vascular endothelial growth factor (VEGF), and were dissociated with Reagent Pack™ Subculture Reagents (Lonza, Cat No. CC-5034). The cells were preincubated with Human BD Fc Block™ (Cat. No. 564219) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histograms) or PE Mouse Anti-Human VEGFR2 (CD309) antibody (Cat. No. 571194/571200; solid line histograms). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing VEGFR2 (CD309) expression (or Ig Isotype control staining) were derived from gated events with the light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: CD309; FLK-1; FLK1; KDR; VEGFR-2; VEGFR2

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human VEGFR-2

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 3791

RRID: AB_3686322

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

Human BD Fc Block™ RUO

Size 50 µg Cat No. 564219

DAPI Solution RUO

Size 1 mg Cat No. 564907

571194 Rev. 1

Antibody Details

BB13-1044

The BB13-1044 monoclonal antibody specifically recognizes Vascular endothelial growth factor receptor 2 (VEGFR-2) which is also known as CD309 and Fetal liver kinase 1 (FLK-1). This protein receptor tyrosine kinase is an ~230 kDa single pass type I transmembrane glycoprotein that is encoded by KDR (Kinase insert domain receptor). It is variably expressed by mesodermal and endothelial cells in embryonic tissues and by vascular endothelial cells, megakaryocytes, platelets, retinal progenitors, and smooth muscle cells in normal adult or tumor tissues. The N-terminal extracellular region of CD309 (VEGFR-2) consists of seven IgC2-like domains which are followed by a single transmembrane region and a C-terminal intracellular tyrosine kinase. The first three extracellular domains mediate ligand binding whereas the remaining four are involved in receptor dimerization. CD309 (VEGFR-2) functions as a receptor for several Vascular endothelial growth factor (VEGF) isoforms. Following receptor dimerization upon ligand binding, CD309 (VEGFR-2) autophosphorylates and stimulates the proliferation and migration of endothelial and other cell types.

571194 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

571194 Rev.1

Citations & References

View product citations for antibody "571194" on CiteAb

Development References (3)

Ferrara N, Houck K, Jakeman L, Leung DW. Molecular and biological properties of the vascular endothelial growth factor family of proteins. Endocr Res. 1992; 13(1):18-32. (Biology). View Reference
Yang L, Soonpaa MH, Adler ED, et al. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature. 2008; 453(7194):524-528. (Biology). View Reference
Ziegler BL, Valtieri M, Porada GA, et al. KDR receptor: a key marker defining hematopoietic stem cells. Science. 1999; 285:1553-1558. (Biology). View Reference

571194 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.