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BD Pharmingen™ PE Mouse Anti-Human CD37
Clone M-B371 (RUO)
Flow cytometric analysis of CD37 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD37 antibody (Cat. No. 561546; solid line histogram) or with a PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD37 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD37 antibody (Cat. No. 561546; solid line histogram) or with a PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
GP52-40; Tetraspanin-26; TSPAN26; Tspan-26
Human (QC Testing)
Mouse BALB/c IgG1, κ
Flow cytometry (Routinely Tested)
5 µl
V CD37.4
AB_10895573
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
PE Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555749
561546 Rev. 1
Antibody Details
M-B371
The M-B371 monoclonal antibody specifically recognizes CD37. CD37 is a 40-52 kDa type II integral membrane glycoprotein present on B cells from the pre-B-cell stage but not on plasma cells. CD37 is also expressed on activated, proliferating cells of germinal centers. There is low expression of CD37 on some T and myeloid cells. The function of CD37 has not yet been clearly identified.
561546 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
561546 Rev.1
Citations & References
View product citations for antibody "561546" on CiteAb
Development References (2)
-
Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
561546 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.