Flow cytometric analysis of CD199 (CCR9) expressed on MOLT-4 cells. Human MOLT-4 cells were stained with either PE Mouse anti-Human CD199 (CCR9) antibody (Cat. No. 561607, solid line histogram) or a PE mIgG2a, κ Isotype Control (Cat. No. 553457; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
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Flow cytometric analysis of CD199 (CCR9) expressed on MOLT-4 cells. Human MOLT-4 cells were stained with either PE Mouse anti-Human CD199 (CCR9) antibody (Cat. No. 561607, solid line histogram) or a PE mIgG2a, κ Isotype Control (Cat. No. 553457; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Product Details
BD Pharmingen™
CDw199; Chemokine (C-C motif) receptor 9; GPR-9-6; GPR28
Human (QC Testing)
Mouse IgG2a
Human CCR9 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
VIII 80134
AB_10895581
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
553457
561607 Rev. 2
Antibody Details
112509
The 112509 monoclonal antibody specifically binds to the human CCR9 chemokine receptor. CCR9 is a thymus specific CC chemokine receptor expressed at high levels by essentially all CD4+ and CD8+ T lymphocytes. CCR9 and its ligand CCL25/TECK are involved in lymphocyte recruitment in the small intestine. The immunogen used to generate the 112509 hybridoma was human CCR9-transfected cells.
561607 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561607 Rev.2
Citations & References
View product citations for antibody "561607" on CiteAb
Development References (4)
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Kunkel EJ, Campbell JJ, Haraldsen G, et al. Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: Epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity. J Exp Med. 2000; 192(5):761-768. (Biology). View Reference
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Kunkel EJ, Kim CH, Lazarus NH, et al. CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells.. J Clin Invest. 2003; 111(7):1001-1010. (Biology). View Reference
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Zaballos A, Gutierrez J, Varona R, Ardavin C, Marquez G. Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol. 1999; 162(10):5671-5675. (Biology). View Reference
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Zabel BA, Agace WW, Campbell JJ, et al. Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis.. J Exp Med. 1999; 190(9):1241-1256. (Biology). View Reference
561607 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.