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      PE Mouse anti-GATA4
      PE Mouse anti-GATA4
      Flow cytometric analysis of PE anti-GATA4 in mouse testis, embryonal carcinoma F9 cells. The F9 cells were either treated with retinoic acid/dbcAMP (solid line) or untreated (dashed line). F9 cells were fixed (BD Cytofix™ Buffer Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Perm Buffer™ III (Cat. No. 558050) on ice for 30 minutes. The cells were stained with PE anti-GATA4 and incubated in the dark for 30 minutes at room temperature. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system. This antibody also works in BD Phosflow™ Perm Buffer I and II.
      PE Mouse anti-GATA4
      Flow cytometric analysis of PE anti-GATA4 in mouse testis, embryonal carcinoma F9 cells. The F9 cells were either treated with retinoic acid/dbcAMP (solid line) or untreated (dashed line). F9 cells were fixed (BD Cytofix™ Buffer Cat. No. 554655) for 10 minutes at 37ºC, permeabilized with BD Perm Buffer™ III (Cat. No. 558050) on ice for 30 minutes. The cells were stained with PE anti-GATA4 and incubated in the dark for 30 minutes at room temperature. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system. This antibody also works in BD Phosflow™ Perm Buffer I and II.
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing), Human (Reported)
      Mouse BALB/c IgG1, κ
      Human GATA4 Peptide
      Intracellular staining (flow cytometry) (Routinely Tested)
      20 µl
      AB_1645187
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      560328 Rev. 2
      Antibody Details
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      L97-56

      The L97-56 monoclonal antibody reacts with GATA4 (GATA-binding protein 4), a member of the GATA family of zinc finger-containing transcription factors that bind to the GATA nucleotide sequence. This ~50 kDa nuclear protein is expressed in mesodermal and definitive endodermal tissues such as the gastrointestinal tract, gonads, and heart. Genetic studies suggest that GATA4 regulates embryonic cardiac development in mice, and disruption of the GATA4 gene leads to defects in heart tube formation; in humans, GATA4 mutations are associated with atrial septal defects. In the adult heart, GATA4 regulates differentiated gene expression. The roles of GATA4 in endocrine and reproductive functions were recently reviewed.

      560328 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 561 nm
      496 nm, 566 nm
      576 nm
      560328 Rev.2
      Citations & References
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      View product citations for antibody "560328" on CiteAb

      Development References (2)

      1. Oka T, Xu J, Molkentin JD. Re-employment of developmental transcription factors in adult heart disease. Semin Cell Dev Biol. 2007; 18(1):117-131. (Biology). View Reference
      2. Viger RS, Guittot SM, Anttonen M, Wilson DB, Heikinheimo M. Role of the GATA family of transcription factors in endocrine development, function, and disease. Mol Endocrinol. 2008; 22(4):781-789. (Biology). View Reference
      560328 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.