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BD Horizon™ PE-CF594 Rat Anti-Mouse CD127
Clone SB/199 (RUO)
Multicolor flow cytometric analysis of CD127 expression on BALB/c mouse splenocytes.
Left Panel: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 561826/553066) and either BD Horizon™ PE-CF594 Rat IgG2b, κ Isotype Control (Cat. No. 562308, dashed line histogram) or with the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Cat. No. 562419, solid line histogram). Histograms (Left Panel) were derived from CD3e-positive gated events with the light scattering characteristics of viable lymphocytes.
Middle and Right Panels: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody and with either a BD Horizon™ PE-CF594 Rat IgG2b, κ isotype control (Middle Panel) or the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Right Panel). Dot plots were derived from gated events based on light scattering characteristics for viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Multicolor flow cytometric analysis of CD127 expression on BALB/c mouse splenocytes.
Left Panel: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 561826/553066) and either BD Horizon™ PE-CF594 Rat IgG2b, κ Isotype Control (Cat. No. 562308, dashed line histogram) or with the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Cat. No. 562419, solid line histogram). Histograms (Left Panel) were derived from CD3e-positive gated events with the light scattering characteristics of viable lymphocytes.
Middle and Right Panels: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody and with either a BD Horizon™ PE-CF594 Rat IgG2b, κ isotype control (Middle Panel) or the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Right Panel). Dot plots were derived from gated events based on light scattering characteristics for viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Multicolor flow cytometric analysis of CD127 expression on BALB/c mouse splenocytes.
Left Panel: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 561826/553066) and either BD Horizon™ PE-CF594 Rat IgG2b, κ Isotype Control (Cat. No. 562308, dashed line histogram) or with the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Cat. No. 562419, solid line histogram). Histograms (Left Panel) were derived from CD3e-positive gated events with the light scattering characteristics of viable lymphocytes.
Middle and Right Panels: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody and with either a BD Horizon™ PE-CF594 Rat IgG2b, κ isotype control (Middle Panel) or the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Right Panel). Dot plots were derived from gated events based on light scattering characteristics for viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Multicolor flow cytometric analysis of CD127 expression on BALB/c mouse splenocytes.
Left Panel: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 561826/553066) and either BD Horizon™ PE-CF594 Rat IgG2b, κ Isotype Control (Cat. No. 562308, dashed line histogram) or with the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Cat. No. 562419, solid line histogram). Histograms (Left Panel) were derived from CD3e-positive gated events with the light scattering characteristics of viable lymphocytes.
Middle and Right Panels: Splenocytes from BALB/c mice were stained with APC Hamster Anti-Mouse CD3e antibody and with either a BD Horizon™ PE-CF594 Rat IgG2b, κ isotype control (Middle Panel) or the BD Horizon™ PE-CF594 Rat Anti-Mouse CD127 antibody (Right Panel). Dot plots were derived from gated events based on light scattering characteristics for viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Product Details
BD Horizon™
Interleukin-7 receptor alpha chain; IL-7R alpha; IL-7RA; IL-7Rα; Il7r
Mouse (QC Testing)
Rat IgG2b, κ
BALB/c mouse pre-B cell line 1A9
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_11153131
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
Product Notices
- An isotype control should be used at the same concentration as the antibody of interest.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- CF™ is a trademark of Biotium, Inc.
- When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
- Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
PE-CF594 Rat IgG2b, κ Isotype Control RUO
Size
0.1 mg
Cat No.
562308
APC Hamster Anti-Mouse CD3e RUO
Size
25 mg
Cat No.
561826
APC Hamster Anti-Mouse CD3e RUO
Size
0.1 mg
Cat No.
553066
562419 Rev. 1
Antibody Details
SB/199
The SB/199 monoclonal antibody specifically binds to mouse CD127 which is also known as, the Interleukin-7 Receptor alpha chain (IL-7Rα). CD127 associates with CD132 (common γ chain) to form a high-affinity signaling IL-7R complex that mediates the biological effects of IL-7. CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP. CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities. IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR expression upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR+ T cells.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
562419 Rev. 1
Format Details
PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and Yellow-Green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and Yellow-Green (561-nm) lasers and the acceptor dye can be excited by the Green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE-CF594
496 nm, 566 nm
615 nm
562419 Rev.1
Citations & References
View product citations for antibody "562419" on CiteAb
Development References (9)
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Akashi K, Kondo M, Weissman IL. Role of interleukin-7 in T-cell development from hematopoietic stem cells. Immunol Rev. 1998; 165:13-28. (Biology). View Reference
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Brugnera E, Bhandoola A, Cibotti R, et al. Coreceptor reversal in the thymus: signaled CD4+8+ thymocytes initially terminate CD8 transcription even when differentiating into CD8+ T cells. Immunity. 2000; 13(1):59-71. (Biology). View Reference
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Faust EA, Saffran DC, Toksoz D, Williams DA, Witte ON. Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells. J Exp Med. 1993; 177(4):915-923. (Biology). View Reference
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Fujihashi K, Kawabata S, Hiroi T, et al. Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes. Proc Natl Acad Sci U S A. 1996; 93(8):3613-3618. (Biology). View Reference
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Goodwin RG, Friend D, Ziegler SF et al. Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell. 1990; 60(6):941-951. (Biology). View Reference
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Henderson AJ, Narayanan R, Collins L, Dorshkind K. Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells. J Immunol. 1992; 149(6):1973-1979. (Biology). View Reference
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Noguchi M, Nakamura Y, Russell SM, et al. Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor. Science. 1993; 262(5141):1877-1880. (Biology). View Reference
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Peschon JJ, Morrissey PJ, Grabstein KH, et al. Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice. J Exp Med. 1994; 180(5):1955-1960. (Biology). View Reference
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Yamashita Y, Oritani K, Miyoshi EK, Wall R, Bernfield M, Kincade PW. Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes. J Immunol. 1999; 162(10):5940-5948. (Clone-specific: Flow cytometry). View Reference
562419 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.