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FITC Mouse Anti-Mouse I-A[b]
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This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [553551] or for additional support, contact your local applications specialist. Contact Us #
Product Details
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BD Pharmingen™
Aβ[b]; H2-IA[b] beta chain; H-2 IA[b] beta chain
Mouse (QC Testing)
Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ
Mouse C3H.SW Splenocytes
Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For immunohistochemical staining (IHC) of cells expressing I-A[b], we recommend the use of biotinylated AF6-120.1 mAb in our special formulation for immunohistochemistry, Cat. No. 550553.  For IHC of I-A[d] expressing cells, we recommend biotinylated mAb AMS-32.1, Cat. No. 550554.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553605 Rev. 11
Antibody Details
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The 25-9-17 monoclonal antibody specifically recognizes the β chain of the I-A[b] MHC class II alloantigen.  It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[q] haplotypes.  Reactivity with other haplotypes (eg, a, f, g7, k, r, s) has not been observed.  The strain distribution of the antigen recognized by this reagent is similar or identical to that of anti-I-A[d] (Aβ[d]) mAb 34-5-3.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553605 Rev. 11
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
553605 Rev.11
Citations & References
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Development References (4)

  1. Cohn LE, Glimcher LH, Waldmann RA, et al. Identification of functional regions on the I-Ab molecule by site-directed mutagenesis. Proc Natl Acad Sci U S A. 1986; 83(3):747-751. (Clone-specific). View Reference
  2. Ikegami H, Makino S, Harada M, Eisenbarth GS, Hattori M. The cataract Shionogi mouse, a sister strain of the non-obese diabetic mouse: similar class II but different class I gene products. Diabetologia. 1988; 31(4):254-258. (Clone-specific). View Reference
  3. Landias D, Beck BN, Buerstedde JM, et al.. The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants. J Immunol. 1986; 137(9):3002-3005. (Clone-specific). View Reference
  4. Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981; 126(1):317-321. (Immunogen: Cytotoxicity, Flow cytometry). View Reference
View All (4) View Less
553605 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.