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      BV421 Rat Anti-Mouse LY-6G
      BV421 Rat Anti-Mouse LY-6G
      Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes (Left and Center Panels).  BALB/c bone-marrow cells were stained with either BD Horizon™ BV421 Rat Anti-Mouse Ly-6G antibody (Cat. No. 562737; solid line histogram) or BD Horizon™ BV421 rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram).  Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid (Left Panel) or myeloid (Center Panel) cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen (Right Panel). A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G antibody (Cat. No. 562737, pseudo-colored green), BD Horizon™ BV480 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 565631, pseudo-colored blue), and Alexa Fluor® 647 Rat Anti-Mouse CD4 antibody (Cat. No. 557681, pseudo-colored red). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.
      BV421 Rat Anti-Mouse LY-6G
      Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes (Left and Center Panels).  BALB/c bone-marrow cells were stained with either BD Horizon™ BV421 Rat Anti-Mouse Ly-6G antibody (Cat. No. 562737; solid line histogram) or BD Horizon™ BV421 rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram).  Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid (Left Panel) or myeloid (Center Panel) cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System. Immunohistofluorescent analysis of Ly-6G expression by cells within C57BL/6 mouse spleen (Right Panel). A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G antibody (Cat. No. 562737, pseudo-colored green), BD Horizon™ BV480 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 565631, pseudo-colored blue), and Alexa Fluor® 647 Rat Anti-Mouse CD4 antibody (Cat. No. 557681, pseudo-colored red). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.
      Product Details
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      BD Horizon™
      Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG2a, κ
      Ly-6G-transfected EL4J Cell Line
      Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
      0.2 mg/ml
      AB_2737756
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      562737 Rev. 3
      Antibody Details
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      1A8

      The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

      562737 Rev. 3
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      562737 Rev.3
      Citations & References
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      View product citations for antibody "562737" on CiteAb

      Development References (2)

      1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen). View Reference
      2. Fleming TJ, Malek TR. Multiple glycosylphosphatidylinositol-anchored Ly-6 molecules and transmembrane Ly-6E mediate inhibition of IL-2 production. J Immunol. 1994; 153(5):1955-1962. (Biology: Flow cytometry, Immunoprecipitation). View Reference
      562737 Rev. 3

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.