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BD OptiBuild™ BUV395 Mouse Anti-Human TSLP Receptor
Clone 1F11/TSLPR (also known as 1F11 and AB81_85.1F11)
(RUO)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
Companion Products
The 1F11/TSLPR monoclonal antibody specifically binds to Thymic Stromal Lymphopoietin Receptor (TSLPR). TSLPR is a member of the hematopoietin receptor superfamily and is also known as Cytokine Receptor-like Factor 2 (CRL2, CRLF2Y, CRLF2). The functional TSLPR complex consists of two subunits, TSLPR and the alpha subunit of the Interleukin-7 Receptor (IL-7Rα). Analysis of the TSLPR reveals sequence similarity with the common cytokine receptor gamma chain (γc; CD132). Functional TSLPRs are expressed by epithelial cells and a variety of hematopoietic cell types, including thymocytes, T cells, B cells, natural killer T cells, monocytes, macrophages, basophils, and dendritic cells (DC). Recent studies indicate that TSLP can activate multiple STAT (Signal Transducer and Activator of Transcription) signaling proteins. TSLP enhances the maturation and viability of DC. It strongly induces DC expression of the CD40 and CD80 costimulatory molecules and chemokines, e.g., TARC (Thymus and activation-regulated chemokine; CCL17) that can attract Th2 effector cells. TSLP supports B cell development. TSLP costimulates the proliferation of naïve T cells in the presence of mature DC. TSLP is also able to increase the sensitivity of T cell receptor-activated CD4+ T cells to low doses of IL-2. In the presence of TSLP, the acute myeloid leukemia-derived cell line, MUTZ-3, shows induced growth and reduced apoptosis. CRLF2 deregulated gene expression is thought to be involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. The 1F11/TSLPR antibody is reportedly a neutralizing antibody.
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.
Development References (10)
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Arima K, Watanabe N, Hanabuchi S, Chang M, Sun SC, Liu YJ. Distinct signal codes generate dendritic cell functional plasticity. Sci Signal. 2010; 3(105):ra4. (Clone-specific: Neutralization). View Reference
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Ito T, Liu YJ, Arima K. Cellular and molecular mechanisms of TSLP function in human allergic disorders--TSLP programs the "Th2 code" in dendritic cells. Allergol Int. 2012; 61(1):35-43. (Biology). View Reference
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Lu N, Wang YH, Arima K, Hanabuchi S, Liu YJ. TSLP and IL-7 use two different mechanisms to regulate human CD4+ T cell homeostasis. J Exp Med. 2009; 206(10):2111-2119. (Biology). View Reference
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Pedroza-Gonzalez A, Xu K, Wu TC, et al. Thymic stromal lymphopoietin fosters human breast tumor growth by promoting type 2 inflammation. J Exp Med. 2011; 208(3):479-490. (Clone-specific: Neutralization). View Reference
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Quentmeier H, Drexler HG, Fleckenstein D, et al. Cloning of human thymic stromal lymphopoietin (TSLP) and signaling mechanisms leading to proliferation. Leukemia. 2001; 15(8):1286-1292. (Biology). View Reference
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Reche PA, Soumelis V, Gorman DM, et al. Human thymic stromal lymphopoietin preferentially stimulates myeloid cells. J Immunol. 2001; 167(1):336-343. (Biology). View Reference
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Rochman I, Watanabe N, Arima K, Liu YJ, Leonard WJ. Cutting edge: direct action of thymic stromal lymphopoietin on activated human CD4+ T cells. J Immunol. 2007; 178(11):6720-6724. (Biology). View Reference
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Russell LJ, Capasso M, Vater I, et al. Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. Blood. 2009; 114(13):2688-2698. (Biology). View Reference
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Tedla N, Bandeira-Melo C, Tassinari P, et al. Activation of human eosinophils through leukocyte immunoglobulin-like receptor 7. Proc Natl Acad Sci U S A. 2003; 100(3):1174-1179. (Biology). View Reference
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Ziegler SF. Thymic stromal lymphopoietin and allergic disease. J Allergy Clin Immunol. 2012; 130(4):845-852. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.