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BD Horizon™ BUV395 Mouse Anti-Human CD90
Clone 5E10 (RUO)
Flow cytometric analysis of CD90 expression on human HEL cells. HEL cells (Human erythroleukemia cell line; ATCC TIB-180) were stained with either BD Horizon™ BUV395 Mouse Anti-Human CD90 antibody (Cat. No. 563804; solid line histogram) or BD Horizon™ BUV395 Mouse IgG1, κ Isotype Control (Cat. No. 563547; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
Flow cytometric analysis of CD90 expression on human mesenchymal stem cells. Human mesenchymal stem cells (Lonza) at passage 7 were harvested using BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527) and stained with either BD Horizon™ BUV395 Mouse anti-Human CD90 antibody (Cat. No. 563804; solid line histogram) or BD Horizon™ BUV395 Mouse IgG1, κ Isotype Control (Cat. No. 563547, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using aBD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD90 expression on human HEL cells. HEL cells (Human erythroleukemia cell line; ATCC TIB-180) were stained with either BD Horizon™ BUV395 Mouse Anti-Human CD90 antibody (Cat. No. 563804; solid line histogram) or BD Horizon™ BUV395 Mouse IgG1, κ Isotype Control (Cat. No. 563547; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
Flow cytometric analysis of CD90 expression on human mesenchymal stem cells. Human mesenchymal stem cells (Lonza) at passage 7 were harvested using BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527) and stained with either BD Horizon™ BUV395 Mouse anti-Human CD90 antibody (Cat. No. 563804; solid line histogram) or BD Horizon™ BUV395 Mouse IgG1, κ Isotype Control (Cat. No. 563547, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using aBD LSRFortessa™ Cell Analyzer System.
Product Details
BD Horizon™
THY1; Thy-1 antigen; Thy-1 membrane glycoprotein
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Pig, Dog (Tested in Development)
Mouse BALB/c IgG1, κ
Human HEL Cell Line
Flow cytometry (Routinely Tested)
5 µl
V M07, BP222; VI BP28, E046
AB_2632398
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- mTESR™1 is a trademark of StemCell Technologies.
- Accutase is a registered trademark of Innovative Cell Technologies, Inc.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
BUV395 Mouse IgG1, k Isotype Control RUO
Size
50 µg
Cat No.
563547
Accutase™ Cell Detachment Solution RUO
Size
100 ML
Cat No.
561527
563804 Rev. 1
Antibody Details
5E10
The 5E10 monoclonal antibody specifically binds to human CD90 which is also known as Thy-1. CD90 is a 25-35 kDa glycophosphatidylinositol-anchored membrane glycoprotein of the Ig superfamily that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. The anti-CD90 antibody binds to a subset of immature CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is highly enriched for cells capable of long-term culture. The anti-CD90 antibody is useful for enriching high proliferative potential colony-forming cells (HIPP-CFC) that are primative progenitor cells.
The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355 nm laser equipped instrument. With an Ex Max at 348 nm and an Em Max at 395 nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.
563804 Rev. 1
Format Details
BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BUV395
348 nm
395 nm
563804 Rev.1
Citations & References
View product citations for antibody "563804" on CiteAb
Development References (6)
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Baum CM, Weissman IL, Tsukamoto AS, Buckle AM, Peault B. Isolation of a candidate human hematopoietic stem-cell population. Proc Natl Acad Sci U S A. 1992; 89(7):2804-2808. (Biology). View Reference
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Craig W, Kay R, Cutler RL, Lansdorp PM. Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med. 1993; 177(5):1331-1342. (Immunogen: Flow cytometry, Immunoprecipitation, Western blot). View Reference
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Lansdorp PM, Thomas TE. AP Gee, ed. Bone Marrow Processing and Purging. Boca Raton FL: CRC Press; 1991.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
563804 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.