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      APC Mouse Anti-Human MIP-1α (CCL3)
      APC Mouse Anti-Human MIP-1α (CCL3)
      Flow cytometric analysis of MIP-1α (CCL3) expression on stimulated Rhesus macaque (Macaca mulatta) peripheral blood monocytes. Rhesus PBMC were stimulated with rhIFN-ɣ (10 ng/ml, 2 hours), then LPS (1 µg/ml, 2 hours) in the presence of GolgiStop™ (Cat. No. 554724; aka monensin 2 µM). The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody (Cat. No. 555397), fixed, permeabilized, and subsequently stained with APC Mouse Anti-Human MIP-1α (CCL3) (Cat. No. 551533). Two-color dot plot was derived from gated events with the forward and side-scattering characteristics of viable monocytes.
      APC Mouse Anti-Human MIP-1α (CCL3)
      Flow cytometric analysis of MIP-1α (CCL3) expression on stimulated Rhesus macaque (Macaca mulatta) peripheral blood monocytes. Rhesus PBMC were stimulated with rhIFN-ɣ (10 ng/ml, 2 hours), then LPS (1 µg/ml, 2 hours) in the presence of GolgiStop™ (Cat. No. 554724; aka monensin 2 µM). The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody (Cat. No. 555397), fixed, permeabilized, and subsequently stained with APC Mouse Anti-Human MIP-1α (CCL3) (Cat. No. 551533). Two-color dot plot was derived from gated events with the forward and side-scattering characteristics of viable monocytes.
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      BD Pharmingen™
      CCL3
      Rhesus, Cynomolgus (QC Testing), Human (Tested in Development)
      Mouse IgG2a, κ
      Intracellular staining (flow cytometry) (Routinely Tested)
      20 µl
      AB_2071051
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      551533 Rev. 3
      Antibody Details
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      11A3

      The 11A3 monoclonal antibody specifically recognizes macrophage inflammatory protein-1α (MIP-1α), a member of the CC chemokine family of proteins. It is produced by T cells, B cells, Langerhans cells, neutrophils and macrophages. MIP-1α plays a role as an inhibitor of stem cell proliferation and as a chempattractant of B cells, eosinophils and cytotoxic T cells. Clone 11A3 also cross-reacts with an intracellular component of rhesus and cynomolgus macaque-LPS-stimulated peripheral blood monocytes. The reactivity pattern observed on CD14-positive cells is similar to that seen on normal human peripheral blood monocytes.

      551533 Rev. 3
      Format Details
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      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      551533 Rev.3
      Citations & References
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      View product citations for antibody "551533" on CiteAb

      Development References (3)

      1. Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). View Reference
      2. Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p.
      3. Wolpe SD, Cerami A. Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J. 1989; 3(14):2565-2573. (Biology). View Reference
      551533 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.