APC Mouse Anti-Human IL-1ra

BD Pharmingen™ APC Mouse Anti-Human IL-1ra

Name: APC Mouse Anti-Human IL-1ra
Brand: APC Mouse Anti-Human IL-1ra
SKU: 570735

Clone AS17 (RUO)

Multiparameter flow cytometric analysis of IL-1ra expression in stimulated Human PBMC. Human PBMC were cultured with Recombinant Human IFN-γ protein (Cat. No. 554617 at 20 ng IFN-γ/ml for 2h; 37°C, Cat. No. 554616/554617) and then lipopolysaccharide (LPS) [1 µg LPS/ml for 24 h; 37°C] was added with Protein Transport Inhibitor (BD GolgiStop™) [Cat. No. 554724]. Cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and then washed and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained in BD Perm/Wash™ Buffer with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plot) or APC Mouse Anti-Human IL-1ra antibody (Cat. No. 570735/570736; Right Plot). The pseudocolor density plot showing the correlated expression of IL-1ra (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals was derived from gated events with the forward and side-light scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: IL-1RN; IL-1ra; IL1 inhibitor; IL1F3; IL1RN; IRAP; sIL-1ra; icIL-1ra

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Recombinant Human IL-1 Receptor Antagonist Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 3557

RRID: AB_3686009

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Fixation Buffer RUO

Size 100 mL Cat No. 554655

Perm/Wash Buffer RUO

Size 100 mL Cat No. 554723

Protein Transport Inhibitor (Containing Monensin) RUO

Size 0.7 mL Cat No. 554724

570735 Rev. 1

Antibody Details

AS17

The AS17 monoclonal antibody specifically recognizes the Interleukin-1 receptor antagonist (IL-1ra or IL-1Ra) which is also known as IL-1 receptor antagonist protein (IRAP), IL-1F3, IL-1RN, and IL1 inhibitor. IL-1ra is an ~17-25 kDa glycoprotein that is encoded by IL1RN. Variants of IL-1ra include a secreted form, sIL-1ra, that is produced by a variety of cell types including activated monocytes, macrophages, neutrophils, and keratinocytes. Intracellular variants of IL-1ra (icIL-1ra) that remain in the cytoplasm have also been described. These include icIL-1Ra1 that is expressed in keratinocytes and other epithelial cells, endothelial cells, fibroblasts, and macrophages. The icIL-1Ra3 variant is expressed in neutrophils and hepatocytes. icIL-1ra proteins may become functional after their release from dead or dying cells although there are indications for their functions inside cells as well. IL-1ra binds to Interleukin-1 Receptor type I (IL-1R1, also known as CD121a) and prevents IL-1α or IL-1β from binding to IL-1R1. IL-1R1 is part of the heterodimeric IL-1 Receptor signaling complex that is comprised of IL-1R1 and the IL-1R accessory protein (IL-1RAcP). icIL-1Ra1 binds to IL-1R1 avidly whereas icIL-1Ra3 binds weakly. IL-1ra does not bind to IL-1RAcP. IL-1ra also binds with low affinity to the nonsignaling Interleukin-1 Receptor type II (IL-1R2, also known as CD121b). IL-1R2 also helps regulate IL-1-mediated activities by acting as a decoy receptor for IL-1 on the membrane of cells or in a soluble form. IL-1ra plays key regulatory roles in health as well as in a variety of diseases including sepsis, colitis, and rheumatoid arthritis.

570735 Rev. 1

Format Details

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 660 nm

570735 Rev.1

Citations & References

View product citations for antibody "570735" on CiteAb

Development References (13)

Arend WP, Joslin FG, Thompson RC, Hannum CH. An IL-1 inhibitor from human monocytes. Production and characterization of biologic properties.. J Immunol. 1989; 143(6):1851-8. (Biology). View Reference
Arend WP, Malyak M, Guthridge CJ, Gabay C. Interleukin-1 receptor antagonist: role in biology.. Annu Rev Immunol. 1998; 16:27-55. (Biology). View Reference
Arend WP, Palmer G, Gabay C. IL-1, IL-18, and IL-33 families of cytokines.. Immunol Rev. 2008; 223:20-38. (Clone-specific). View Reference
Dinarello CA, van der Meer JW. Treating inflammation by blocking interleukin-1 in humans.. Semin Immunol. 2013; 25(6):469-84. (Biology). View Reference
Dinarello CA. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases.. Blood. 2011; 117(14):3720-32. (Biology). View Reference
Hannum CH, Wilcox CJ, Arend WP, et al. Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor.. Nature. 1990; 343(6256):336-40. (Biology). View Reference
Haskill S, Martin G, Van Le L, et al. cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium.. Proc Natl Acad Sci U S A. 1991; 88(9):3681-5. (Biology). View Reference
Janson RW, Hance KR, Arend WP. Production of IL-1 receptor antagonist by human in vitro-derived macrophages. Effects of lipopolysaccharide and granulocyte-macrophage colony-stimulating factor.. J Immunol. 1991; 147(12):4218-23. (Biology). View Reference
Kennedy MC, Rosenbaum JT, Brown J, et al. Novel production of interleukin-1 receptor antagonist peptides in normal human cornea.. J Clin Invest. 1995; 95(1):82-8. (Biology). View Reference
Krzesicki RF, Hatfield CA, Bienkowski MJ, et al. Regulation of expression of IL-1 receptor antagonist protein in human synovial and dermal fibroblasts.. J Immunol. 1993; 150(9):4008-18. (Biology). View Reference
McColl SR, Paquin R, Ménard C, Beaulieu AD. Human neutrophils produce high levels of the interleukin 1 receptor antagonist in response to granulocyte/macrophage colony-stimulating factor and tumor necrosis factor alpha.. J Exp Med. 1992; 176(2):593-8. (Biology). View Reference
Rhind SG, Castellani JW, Brenner IK, et al. Intracellular monocyte and serum cytokine expression is modulated by exhausting exercise and cold exposure.. Am J Physiol Regul Integr Comp Physiol. 2001; 281(1):R66-75. (Clone-specific: Intracellular Staining/Flow Cytometry). View Reference
Vamvakopoulos J, Green C, Metcalfe S. Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.. Eur J Immunol. 2002; 32(10):2988-96. (Clone-specific: Intracellular Staining/Flow Cytometry). View Reference

View All (13)

570735 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.