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      Alexa Fluor™ 647 Mouse Anti-Human IL-33 Receptor (ST2)
      Alexa Fluor™ 647 Mouse Anti-Human IL-33 Receptor (ST2)

      Flow cytometric analysis of IL-33 Receptor (ST2) expression on KU812 cells.  Cells from the Human KU812 (Chronic Myelogenous Leukemia, ATCC® CRL-2099™) cell line were cultured in the presence (Left Plot) or absence of serum (Right Plot) for 6 days. The cells were then preincubated with Human BD Fc Block™ (Cat. No. 564219/564220) and stained with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histograms) or Alexa Fluor™ 647 Mouse Anti-Human IL-33 Receptor (ST2) antibody (Cat. No. 569428/569429; solid line histograms). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor (ST2) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

      Alexa Fluor™ 647 Mouse Anti-Human IL-33 Receptor (ST2)

      Flow cytometric analysis of IL-33 Receptor (ST2) expression on KU812 cells.  Cells from the Human KU812 (Chronic Myelogenous Leukemia, ATCC® CRL-2099™) cell line were cultured in the presence (Left Plot) or absence of serum (Right Plot) for 6 days. The cells were then preincubated with Human BD Fc Block™ (Cat. No. 564219/564220) and stained with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histograms) or Alexa Fluor™ 647 Mouse Anti-Human IL-33 Receptor (ST2) antibody (Cat. No. 569428/569429; solid line histograms). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing IL-33 Receptor (ST2) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

      Product Details
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      BD Pharmingen™
      IL1RL1; IL33R; ST2; interleukin-1 receptor-like 1
      Human (QC Testing)
      Mouse IgG1, κ
      Human IL-33R Recombinant Protein
      Flow cytometry (Routinely Tested)
      5 µl/test
      9173
      AB_3685056
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      11. For U.S. patents that may apply, see bd.com/patents.
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      569428 Rev. 3
      Antibody Details
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      U33-374

      The U33-374 monoclonal antibody specifically binds to the Human Interleukin-33 Receptor (ST2), which is also known as IL-33 Receptor, IL-33R, IL1RL1 or Interleukin-1 receptor-like 1. The IL-33 Receptor (ST2) exists in either a type I transmembrane or soluble glycoprotein form. These IL-33 Receptor (ST2) forms are encoded by the IL1RL1 (Interleukin-1 receptor-like 1) gene which belongs to the IL-1 Receptor family within the Ig superfamily. The IL-33 Receptor (ST2) is expressed by subsets of T cells, including Th2-like cells and some regulatory T cells, as well as some innate lymphocytes, eosinophils, basophils, and mast cells. The IL-33 Receptor (ST2) binds IL-33 and complexes with the IL-1R Accessory Protein (IL1RAP) to form a functional signaling receptor complex that can induce the production of T helper type 2 (Th2) cytokines. The soluble IL-33 Receptor (ST2) may function as a decoy receptor which can block the binding of IL-33 to the transmembrane IL-33R. The IL-33 Receptor (ST2) plays roles in inflammation, immunity, and allergy.

      569428 Rev. 3
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      569428 Rev.3
      Citations & References
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      View product citations for antibody "569428" on CiteAb

      Development References (11)

      1. Allakhverdi Z, Smith DE, Comeau MR, Delespesse G. Cutting edge: The ST2 ligand IL-33 potently activates and drives maturation of human mast cells.. J Immunol. 2007; 179(4):2051-4. (Biology). View Reference
      2. Blom L, Poulsen LK. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.. J Immunol. 2012; 189(9):4331-7. (Biology). View Reference
      3. Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance.. Nat Immunol. 2004; 5(4):373-9. (Biology). View Reference
      4. Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.. J Allergy Clin Immunol. 2008; 121(6):1484-90. (Biology). View Reference
      5. Chow JY, Wong CK, Cheung PF, Lam CW. Intracellular signaling mechanisms regulating the activation of human eosinophils by the novel Th2 cytokine IL-33: implications for allergic inflammation.. Cell Mol Immunol. 2010; 7(1):26-34. (Biology). View Reference
      6. Griesenauer B, Paczesny S. The ST2/IL-33 Axis in Immune Cells during Inflammatory Diseases.. Front Immunol. 2017; 8:475. (Biology). View Reference
      7. Jo S, Kim E, Kwak A, et al. Reconstitution of ST2 (IL-1R4) specific for IL-33 activity; no suppression by IL-1Ra though a common chain IL-1R3 (IL-1RAcP) shared with IL-1.. Cytokine. 2016; 83:33-40. (Biology). View Reference
      8. Liew FY, Pitman NI, McInnes IB. Disease-associated functions of IL-33: the new kid in the IL-1 family.. Nat Rev Immunol. 2010; 10(2):103-10. (Biology). View Reference
      9. Pecaric-Petkovic T, Didichenko SA, Kaempfer S, Spiegl N, Dahinden CA. Human basophils and eosinophils are the direct target leukocytes of the novel IL-1 family member IL-33.. Blood. 2009; 113(7):1526-34. (Biology). View Reference
      10. Suzukawa M, Koketsu R, Iikura M, et al. Interleukin-33 enhances adhesion, CD11b expression and survival in human eosinophils.. Lab Invest. 2008; 88(11):1245-53. (Biology). View Reference
      11. Wong CK, Leung KM, Qiu HN, Chow JY, Choi AO, Lam CW. Activation of eosinophils interacting with dermal fibroblasts by pruritogenic cytokine IL-31 and alarmin IL-33: implications in atopic dermatitis.. PLoS One. 2012; 7(1):e29815. (Biology). View Reference
      View All (11) View Less
      569428 Rev. 3

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.