Multiparameter flow cytometric analysis of CD352 (NTB-A/SLAMF6) expression on human peripheral blood leucocytes. Whole blood cells were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357, Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD352 (NTB-A/SLAMF6) antibody (Cat. No. 566093; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of CD352 (NTB-A/SLAMF6) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Multiparameter flow cytometric analysis of CD352 (NTB-A/SLAMF6) expression on human peripheral blood leucocytes. Whole blood cells were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357, Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD352 (NTB-A/SLAMF6) antibody (Cat. No. 566093; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of CD352 (NTB-A/SLAMF6) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
SLAMF6; SLAF6; NTBA; NTB-A; NK-T-B-antigen; KALI; SF2000; Ly108
Human (QC Testing)
Mouse IgG2a, κ
Human NTB-A/SLAMF6 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
IX 4
AB_2739497
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
50 µg
Cat No.
565357
566093 Rev. 1
Antibody Details
292811
The 292811 monoclonal antibody specifically binds to CD352 which is also known as SLAM family member 6 (SLAMF6), NK-T-B-antigen (NTB-A), or Activating NK receptor. CD352 is a ~60 kDa type I transmembrane glycoprotein that contains two ITSMs (immunoreceptor tyrosine-based switch motifs) in its cytoplasmic domain and belongs to the CD2 subfamily of the immunoglobulin superfamily. CD352 is expressed by thymocytes, NK cells, B cells, and T cells. CD352 functions through homophilic interactions as a coreceptor for the activation of NK cells and lymphocytes. It plays roles in regulating various cellular effector functions including cytolytic activity, production of cytokines such as IFN-γ and IL-17, and immunoglobulin class switching.
566093 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566093 Rev.1
Citations & References
View product citations for antibody "566093" on CiteAb
Development References (6)
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Chatterjee M, Hedrich CM, Rauen T, Ioannidis C, Terhorst C, Tsokos GC. CD3-T cell receptor co-stimulation through SLAMF3 and SLAMF6 receptors enhances RORγt recruitment to the IL17A promoter in human T lymphocytes.. J Biol Chem. 2012; 287(45):38168-77. (Biology). View Reference
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Eissmann P, Watzl C.. Molecular analysis of NTB-A signaling: a role for EAT-2 in NTB-A-mediated activation of human NK cells.. J Immunol. 2006; 177(5):3170-3177. (Biology). View Reference
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Flaig RM, Stark S, Watzl C.. Cutting edge: NTB-A activates NK cells via homophilic interaction. J Immunol. 2004; 172(11):6424-6427. (Biology). View Reference
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Kumar KR, Li L, Yan M, et al. Regulation of B cell tolerance by the lupus susceptibility gene Ly108. Science. 2006; 312(5780):1606-1608. (Biology). View Reference
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Ramirez PW, DePaula-Silva AB, Szaniawski M, Barker E, Bosque A, Planelles V. HIV-1 Vpu utilizes both cullin-RING ligase (CRL) dependent and independent mechanisms to downmodulate host proteins.. Retrovirology. 2015; 12:65. (Biology). View Reference
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Valdez PA, Wang H, Seshasayee D, et al. NTB-A, a new activating receptor in T cells that regulates autoimmune disease.. J Biol Chem. 2004; 279(18):18662-18669. (Biology). View Reference
566093 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.