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      Alexa Fluor® 488 Mouse anti-Human Granulysin
      Alexa Fluor® 488 Mouse anti-Human Granulysin
      Flow cytometric analysis of anti-human granulysin on human peripheral blood mononuclear cells (PBMC).  Isolated human PBMC were stained with APC anti-human CD56 (clone B159, Cat. No. 555518).  Cells were then fixed and permeabilized with BD Cytofix/Cytoperm™ (Cat. No. 554722) followed by staining with either Alexa 488 anti-granulysin (clone RB1, Cat. No. 558254, right panel) or a Alexa 488 conjugated isotype control (clone MOPC-21; Cat. No. 557702, left panel).  Flow cytometry was performed on a BD FACSCalibur™ System.
      Alexa Fluor® 488 Mouse anti-Human Granulysin
      Flow cytometric analysis of anti-human granulysin on human peripheral blood mononuclear cells (PBMC).  Isolated human PBMC were stained with APC anti-human CD56 (clone B159, Cat. No. 555518).  Cells were then fixed and permeabilized with BD Cytofix/Cytoperm™ (Cat. No. 554722) followed by staining with either Alexa 488 anti-granulysin (clone RB1, Cat. No. 558254, right panel) or a Alexa 488 conjugated isotype control (clone MOPC-21; Cat. No. 557702, left panel).  Flow cytometry was performed on a BD FACSCalibur™ System.
      Product Details
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      BD Pharmingen™
      GNLY; LAG2; lymphocyte-activation gene 2; NKG5; TLA519; T-cell activation protein 519
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human Granulysin
      Intracellular staining (flow cytometry) (Routinely Tested)
      20 µl
      AB_2869123
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
      6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      7. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558254 Rev. 4
      Antibody Details
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      RB1

      The monoclonal antibody RB1 recognizes both 15-kDa precursor and 9-kDa effector forms of human granulysin, a protein present in granules of human natural killer (NK) cells and activated cytotoxic T lymphocytes (CTLs). Granulysin is cytolytic against both tumor and microbial targets and has been found to kill extracellular and intracellular mycobacterium tuberculosis through a granule-dependent mechanism.  Granulysin is a member of a larger family of proteins known as saposin-like proteins (SAPLIP) that are thought to associate with lipids. The crystal structure of granulysin suggests that it functions as a lytic molecule. To date, no granulysin homolog has been found in mice, precluding the generation of granulysin knockout animals.

      This product is sold under license from MBL, Inc.

      558254 Rev. 4
      Format Details
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      Alexa Fluor™ 488
      Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 488
      Blue 488 nm
      494 nm
      517 nm
      558254 Rev.4
      Citations & References
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      View product citations for antibody "558254" on CiteAb

      Development References (4)

      1. Clayberger C, Krensky AM. Granulysin. Curr Opin Immunol. 2003; 15(5):560-5. (Biology). View Reference
      2. Deng A, Chen S, Li Q, Lyu SC, Clayberger C, Krensky AM. Granulysin, a cytolytic molecule, is also a chemoattractant and proin flammatory activator. J Immunol. 2005; 174:5243-48. (Biology). View Reference
      3. Ogawa K, Takamori Y, Suzuki K, et al. Granulysin in human serum as marker of cell mediated immunity. Eur J Immunol. 2003; 33:1925-1933. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). View Reference
      4. Pena SV, Krensky AM. Granulysin, a new human cytolytic granule-associated protein with possible involvement in cell-mediated cytotoxicity. Semin Immunol. 1997; 9(2):117-125. (Biology). View Reference
      View All (4) View Less
      558254 Rev. 4

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.