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      Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control
      Product Details
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      BD Pharmingen™
      Mouse BALB/c IgG2a, κ
      None
      Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
      0.2 mg/ml
      AB_10563084
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      An immunoglobulin isotype control should be used at the same concentration as the antibody of interest.

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      Product Notices

      1. This antibody has been developed for the application listed above. However, a routine test is not performed on every lot.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Alexa Fluor® 700 has an adsorption maximum of ~700nm and a peak fluorescence emission of ~720nm. Before staining cells with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
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      560894 Rev. 1
      Antibody Details
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      MOPC-173

      The MOPC-173 antibody has unknown specificity.  The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.  In the absence of antigen-specific binding, this immunoglobulin may bind non-specifically to Fc receptors.

      560894 Rev. 1
      Format Details
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      Alexa Fluor™ 700
      Alexa Fluor™ 700 dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 697 nm and an emission maximum (Em Max) at 719-nm. Alexa Fluor™ 700 is designed to be excited by the Red (627–640-nm) laser and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 700
      Red 627-640 nm
      697 nm
      719 nm
      560894 Rev.1
      Citations & References
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      View product citations for antibody "560894" on CiteAb
      No Citations Found

      No Citations are available for this Product

      560894 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.