Description
The BD IntraSure™ kit is a reagent kit designed to fix and permeabilize cells prior to intracellular immunofluorescence staining with monoclonal antibodies. This formulation enables optimal staining of intracellular targets with minimal effect on existing cell surface staining and cell recovery. Samples are analyzed by flow cytometry.
Preparation And Storage
Store vials at room temperature (20°C to 25°C) and protect from light. Do not freeze. Each reagent is stable for the period shown on the bottle label when stored as directed.
Development References (7)
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Bennett F, Rawstron A, Plummer M, et al. B-cell chronic lymphocytic leukaemia cells show specific changes in membrane protein expression during different stages of cell cycle. Br J Haematol. 2007; 139:600-604. (Biology).
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Dworzak MN, Fritsch G, Fröschl G, Printz D, Gadner H. Four-color flow cytometric investigation of terminal deoxynucleotidyl transferase-positive lymphoid precursors in pediatric bone marrow: CD79a expression precedes CD19 in early B-cell ontogeny.. Blood. 1998; 92(9):3203-9. (Biology). View Reference
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Letestu R, Rawstron A, Ghia P, et al. Evaluation of ZAP-70 expression by flow cytometry in chronic lymphocytic leukemia: A multicentric international harmonization process. Cytometry B Clin Cytom. 2006; 70:309-314. (Biology).
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Nakase K, Sartor M, Bradstock K. Detection of myeloperoxidase by flow cytometry in acute leukemia. Cytometry. 1998; 34:198-202. (Biology).
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de Tute RM, Jack AS, Child JA, Morgan GJ, Owen RG, Rawstron AC. A single-tube six-colour flow cytometry screening assay for the detection of minimal residual disease in myeloma. Leukemia. 2007; 21:2046-2049. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.