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Purified Mouse Anti-AKAP82
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Mouse AKAP82 aa. 555-675
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
82 kDa
250 µg/ml
AB_399010
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611564 Rev. 1
Antibody Details
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25/AKAP82

The cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain the localization of PKA, the regulatory subunits interact with specific anchoring proteins. Several proteins have been identified as PKA anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). Fifteen of the AKAP proteins contain a consensus binding motif that allows interaction with the type II regulatory subunit (RII) of the PKA holoenzyme. In addition, three other AKAPs (D-AKAP1, D-AKAP2, and fsc1/AKAP82) can associate with the type I regulatory subunit (RI) of the PKA holoenzyme. AKAP82 was isolated as a component of the mouse sperm fibrous sheath. It is a dual specificity AKAP that contains an RII-binding domain (domain A; amino acids 219 to 232) and an RI-binding domain (domain B; amino acids 335-344). In mouse, pro-AKAP82 is synthesized as a 97 kDa precursor that is transported to the flagellum where proteolytic cleavage of the N-terminal 179 amino acids produces AKAP82. Assembly of AKAP82 into the fibrous sheath surrounding the axoneme of the sperm flagellum is thought to tether PKA close to the axoneme where it can regulate flagellar motility.

611564 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611564 Rev.1
Citations & References
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Development References (4)

  1. Edwards AS, Scott JD. A-kinase anchoring proteins: protein kinase A and beyond. Curr Opin Immunol. 2000; 12(2):217-221. (Biology). View Reference
  2. Fulcher KD, Mori C, Welch JE, O'Brien DA, Klapper DG, Eddy EM. Characterization of Fsc1 cDNA for a mouse sperm fibrous sheath component. Biol Reprod. 1995; 52(1):41-49. (Biology). View Reference
  3. Miki K, Eddy EM. Identification of tethering domains for protein kinase A type Ialpha regulatory subunits on sperm fibrous sheath protein FSC1. J Biol Chem. 1998; 273(51):34384-34390. (Biology). View Reference
  4. Turner RM, Johnson LR, Haig-Ladewig L, Gerton GL, Moss SB. An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail. J Biol Chem. 1998; 273(48):32135-32141. (Biology). View Reference
View All (4) View Less
611564 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.