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Purified Mouse Anti-4.1N
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken (Tested in Development)
Mouse IgG1
Mouse 4.1N aa. 510-626
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
116 & 100 kDa
250 µg/ml
AB_2098366
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611836 Rev. 1
Antibody Details
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4/4.1N

The erythrocyte membrane cytoskeletal protein, 4.1R, is an important structural element of red blood cells. It contains a spectrin-actin-binding domain (SABD) that facilitates spectrin interaction with F-actin and a membrane-binding domain (MBD) that links the cytoskeleton to the plasma membrane via interactions with Band 3 protein. Two homologues of 4.1R, 4.1G and 4.1N, are expressed throughout the body and in neurons, respectively. These 4.1 proteins contain Ca2+-dependent (CD-CaM) and Ca2+-independent calmodulin (CI-CaM) binding sites within the MBD domain, a centrally located SABD, and a conserved C-terminal domain (CTD). 4.1N expression is high throughout the adult brain and lower in heart, kidney, and pancreas. In neurons, 4.1N protein co-localizes with PSD-95 and GluR1 at sites of synaptic contacts and the CTD of 4.1N allows binding to the C-terminus of GluR1. In addition, 4.1N interacts with the PI3-Kinase enhancer (PIKE) protein and translocates to the nucleus along with PIKE in response to NGF treatment. Thus, 4.1N is a neuronal membrane cytoskeletal protein that may be involved with localizaton of proteins such as neurotransmitter receptors and signaling molecules. Predominant isoforms of 4.1 proteins have been reported to be observable between 30-210 kD due to complex alternative splicing events.  4.1N has been reported to be observable in a range of 100-135 kD in neuronal populations.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611836 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611836 Rev.1
Citations & References
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Development References (3)

  1. Shen L, Liang F, Walensky LD, Huganir RL. Regulation of AMPA receptor GluR1 subunit surface expression by a 4. 1N-linked actin cytoskeletal association. J Neurosci. 2000; 20(21):7932-7940. (Biology). View Reference
  2. Walensky LD, Blackshaw S, Liao D, et al. A novel neuron-enriched homolog of the erythrocyte membrane cytoskeletal protein 4.1. J Neurosci. 1999; 19(15):6457-6467. (Biology). View Reference
  3. Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell. 2000; 103(6):919-930. (Biology). View Reference
611836 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.