Skip to main content Skip to navigation
PKC Sampler Kit
PKC Sampler Kit
IP: nat = native condition, den = denatured conditions Dilutions are recommended based on western blotting on the indicated positive control. This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.
IP: nat = native condition, den = denatured conditions Dilutions are recommended based on western blotting on the indicated positive control. This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Western blot (Routinely Tested)
RUO
AB_2870030


Description

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least ten isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain that contains four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC subfamily members (δ, ε, ζ, η and θ isoforms) are Ca2+ independent and lack the C2 domain. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. Different tissues seem to have their own characteristic patterns of PKC isozyme expression, leading to the hypothesis that each isozyme has a different biological function.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611421 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.