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Purified Mouse Anti-R-Cadherin
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG2a, κ
Mouse R-Cadherin aa. 22-201
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
100 kDa
250 µg/ml
AB_2077787
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610414 Rev. 2
Antibody Details
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48/R-Cadherin

R-Cadherin was first isolated from chicken retina, but was subsequently shown to be present and highly conserved in a variety of tissues from several different species. A 913 amino acid polypeptide, R-Cadherin is highly homologous to Cadherin-4, but is most closely related to another family member, N-Cadherin. In experiments using chimeric L cells expressing chicken or mouse Cadherins, those cells expressing mouse or chicken R-Cadherin were able to aggregate with each other as well as with cells expressing N-Cadherin. However, there was no aggregation of R-Cadherin expressing cells, with P- or E-Cadherin expressing cells. This suggests that the roles of specific cadherins are conserved among types in the determination of heterogenous cell segregation and distribution during tissue morphogenesis and maintenance.

610414 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610414 Rev.2
Citations & References
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Development References (3)

  1. Hutton JC, Christofori G, Chi WY, et al. Molecular cloning of mouse pancreatic islet R-cadherin: differential expression in endocrine and exocrine tissue. Mol Endocrinol. 1993; 7(9):1151-1160. (Biology). View Reference
  2. Matsunami H, Miyatani S, Inoue T, et al. Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene. J Cell Sci. 1993; 106(1):401-409. (Biology). View Reference
  3. Tanihara H, Sano K, Heimark RL, St John T, Suzuki S. Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin. Cell Adhes Commun. 1994; 2(1):15-26. (Biology). View Reference
610414 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.