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Purified Mouse Anti-Lyn
Purified Mouse Anti-Lyn
Western blot analysis of Lyn on HEL (human erythroleukemia cell line) lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Lyn.
Purified Mouse Anti-Lyn

Immunofluorescent staining on Human Fibroblasts

Western blot analysis of Lyn on HEL (human erythroleukemia cell line) lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Lyn.

Immunofluorescent staining on Human Fibroblasts

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Reactivity Confirmed in Development), Chicken (Lack of Reactivity Confirmed in Development)
Mouse IgG1
Human Lyn aa. 1-138
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
56 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610003 Rev. 1
Antibody Details
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42/Lyn

Lyn is a 56 kDa cytosolic tyrosine kinase that belongs to the Src family of kinases. In addition to Src, this family includes lck, Fgr, Hck, Blk, Yes, fyn, and Yrk kinases. The lyn gene was isolated from a human placenta cDNA library and later found in a variety of cell types. Similar to other members of the Src family of tyrosine kinases, Lyn has been implicated in the regulation of cell signaling mechanisms. Lyn's function has primarily been studied in hematopoietic cells where it is associated with the high-affinity IgE receptor in basophils. It also associates with p120 Ras-GAP in thrombin-stimulated platelets. This association corresponds to an increase in Lyn kinase activity. Lyn is often observed as a doublet of about 56 kDa and 53 kDa. These forms may represent alternative splicing products of lyn mRNA.  

610003 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610003 Rev.1
Citations & References
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Development References (5)

  1. Bolen JB, Rowley RB, Spana C, Tsygankov AY. The Src family of tyrosine protein kinases in hemopoietic signal transduction. FASEB J. 1992; 6(15):3403-3409. (Biology). View Reference
  2. Deehan MR, Harnett W, Harnett MM. A filarial nematode-secreted phosphorylcholine-containing glycoprotein uncouples the B cell antigen receptor from extracellular signal-regulated kinase-mitogen-activated protein kinase by promoting the surface Ig-mediated recruitment of Src homology 2 domain-containing tyrosine phosphatase-1 and Pac-1 mitogen-activated kinase-phosphatase. J Immunol. 2001; 166(12):7462-7468. (Clone-specific: Western blot). View Reference
  3. Liang X, Wisniewski D, Strife A, Shivakrupa, Clarkson B, Resh MD. Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling. J Biol Chem. 2002; 277(16):13732-13738. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  4. Liu J, Oh P, Horner T, Rogers RA, Schnitzer JE. Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains. J Biol Chem. 1997; 272(11):7211-7222. (Clone-specific). View Reference
  5. Muller G, Jung C, Wied S, Welte S, Jordan H, Frick W. Redistribution of glycolipid raft domain components induces insulin-mimetic signaling in rat adipocytes. Mol Cell Biol. 2001; 21(14):4553-4567. (Clone-specific: Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610003 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.