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V450 Rat Anti-Mouse CD3 Molecular Complex
V450 Rat Anti-Mouse CD3 Molecular Complex

Flow cytometric analysis of CD3 expression on mouse splenocytes.  Left Panel: Splenocytes from BALB/c mice were stained with either a BD Horizon™ V450 Rat IgG2b, κ Isotype Control (Cat. No. 560457; dashed line histogram) or with the BD Horizon™ V450 Rat anti-Mouse CD3 antibody (Cat. No. 561389; solid line histogram).  Middle and Right Panels: Splenocytes from BALB/c mice were stained with a FITC Rat anti-Mouse CD19 antibody (Cat. No. 553785) in conjunction with either a BD Horizon™ V450 Rat IgG2b, κ Isotype Control (Middle Panel) or the BD Horizon™ V450 Rat anti-Mouse CD3 antibody (Right Panel). The fluorescence histograms and dot plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of CD3 expression on mouse splenocytes.  Left Panel: Splenocytes from BALB/c mice were stained with either a BD Horizon™ V450 Rat IgG2b, κ Isotype Control (Cat. No. 560457; dashed line histogram) or with the BD Horizon™ V450 Rat anti-Mouse CD3 antibody (Cat. No. 561389; solid line histogram).  Middle and Right Panels: Splenocytes from BALB/c mice were stained with a FITC Rat anti-Mouse CD19 antibody (Cat. No. 553785) in conjunction with either a BD Horizon™ V450 Rat IgG2b, κ Isotype Control (Middle Panel) or the BD Horizon™ V450 Rat anti-Mouse CD3 antibody (Right Panel). The fluorescence histograms and dot plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Horizon™
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
γδ TCR-positive T-T hybridoma D1
Flow cytometry (Routinely Tested)
0.2 mg/ml
12501
AB_10679120
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  7. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
561389 Rev. 1
Antibody Details
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17A2

The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

561389 Rev. 1
Format Details
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V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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V450
Violet 405 nm
405 nm
450 nm
561389 Rev.1
Citations & References
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Development References (2)

  1. Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Immunogen: Cytotoxicity, Functional assay, Immunohistochemistry, Immunoprecipitation, Stimulation). View Reference
  2. Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Clone-specific: Depletion). View Reference
561389 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.