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      RY586 Mouse Anti-Human γδ TCR
      RY586 Mouse Anti-Human γδ TCR
      Multiparameter flow cytometric analysis using BD OptiBuild™ RY586 Mouse Anti-Human γδ TCR antibody (Cat. No. 753300) on human peripheral blood lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      RY586 Mouse Anti-Human γδ TCR
      Multiparameter flow cytometric analysis using BD OptiBuild™ RY586 Mouse Anti-Human γδ TCR antibody (Cat. No. 753300) on human peripheral blood lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      Product Details
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      BD OptiBuild™
      TCRgd; γδ TCR; TRG@/TRD@; TCRG/TCRD; TCR gamma delta
      Human (Tested in Development)
      Mouse BALB/c IgG1
      Sepharose® bead/CD3/γ/δ TCR complex
      Flow cytometry (Qualified)
      0.2 mg/ml
      6964, 6965
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      753300 Rev. 2
      Antibody Details
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      11F2

      The 11F2 monoclonal antibody specifically reacts with a framework epitope of the γ/δ TCR. The γ/δ TCR is a heterodimeric glycoprotein that is noncovalently associated with the CD3 antigen complex. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit. TCR γ/δ is present on a minor subset of T lymphocytes in peripheral blood, thymus, spleen, and lymph node. TCR γ/δ-positive T lymphocytes comprise 1% to 9% of normal peripheral blood lymphocytes and less than 2% of normal thymocytes. The 11F2 antibody is mitogenic for γ/δ-TCR-bearing T lymphocytes.

      753300 Rev. 2
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753300 Rev.2
      Citations & References
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      View product citations for antibody "753300" on CiteAb

      Development References (15)

      1. Blink SE, Miller SD. The contribution of gammadelta T cells to the pathogenesis of EAE and MS.. Curr Mol Med. 2009; 9(1):15-22. (Biology). View Reference
      2. Bonneville M, O'Brien RL, Born WK. Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 2110; 10(7):467-478. (Biology). View Reference
      3. Borst J, van Dongen JJ, Bolhuis RL, et al. Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody.. J Exp Med. 1988; 167(5):1625-44. (Immunogen: Electron microscopy, ELISA, Flow cytometry, Fluorescence microscopy, Immunofluorescence). View Reference
      4. Cairo C, Hebbeler AM, Propp N, Bryant JL, Colizzi V, Pauza CD. Innate-like gammadelta T cell responses to mycobacterium Bacille Calmette-Guerin using the public V gamma 2 repertoire in Macaca fascicularis.. Tuberculosis (Edinb). 2007; 87(4):373-83. (Biology). View Reference
      5. Carding SR, Egan PJ. The importance of gamma delta T cells in the resolution of pathogen-induced inflammatory immune responses.. Immunol Rev. 2000; 173:98-108. (Biology). View Reference
      6. Chen ZW. Immune regulation of gammadelta T cell responses in mycobacterial infections.. Clin Immunol. 2005; 116(3):202-7. (Biology). View Reference
      7. García VE, Sieling PA, Gong J, et al. Single-cell cytokine analysis of gamma delta T cell responses to nonpeptide mycobacterial antigens.. J Immunol. 1997; 159(3):1328-35. (Biology). View Reference
      8. Huang D, Chen CY, Zhang M, et al. Clonal immune responses of Mycobacterium-specific γδ T cells in tuberculous and non-tuberculous tissues during M. tuberculosis infection.. PLoS ONE. 2012; 7(2):e30631. (Biology). View Reference
      9. Ichinohasama R, Miura I, Takahashi T, et al. Peripheral CD4+ CD8- gammadelta T cell lymphoma: a case report with multiparameter analyses.. Hum Pathol. 1996; 27(12):1370-7. (Clone-specific: Flow cytometry). View Reference
      10. Lanier LL, Federspiel NA, Ruitenberg JJ, et al. The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.. J Exp Med. 1987; 165(4):1076-94. (Clone-specific: Flow cytometry). View Reference
      11. Lanier LL, Ruitenberg J, Bolhuis RL, Borst J, Phillips JH, Testi R. Structural and serological heterogeneity of gamma/delta T cell antigen receptor expression in thymus and peripheral blood.. Eur J Immunol. 1988; 18(12):1985-92. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
      12. Lanier LL, Serafini AT, Ruitenberg JJ, et al. The gamma T-cell antigen receptor.. J Clin Immunol. 1987; 7(6):429-40. (Biology). View Reference
      13. Testi R, Lanier LL. Functional expression of CD28 on T cell antigen receptor gamma/delta-bearing T lymphocytes.. Eur J Immunol. 1989; 19(1):185-8. (Clone-specific: Flow cytometry, Functional assay). View Reference
      14. Urban EM, Chapoval AI, Pauza CD. Repertoire development and the control of cytotoxic/effector function in human gammadelta T cells.. Clin Dev Immunol. 2010; 2010:732893. (Biology). View Reference
      15. Voogt PJ, Falkenburg JH, Fibbe WE, et al. Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor-/CD3-, T cell receptor gamma delta+/CD3+ and T cell receptor-alpha beta+/CD3+ lymphocytes.. J Immunol. 1989; 142(5):1774-80. (Biology). View Reference
      View All (15) View Less
      753300 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.