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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
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The 3D6 monoclonal antibody specifically recognizes CD169 which is a ~185 kDa type I transmembrane glycoprotein that is encoded by Siglec1. CD169 belongs to the Sialic acid binding Ig like lectins (Siglec) family and is also known as Sialoadhesin (Sn), Siglec-1, or Sheep erythrocyte receptor (SER). CD169 is expressed on subsets of macrophages found in the bone marrow, secondary lymphoid tissues, liver colon and lungs. It binds to the oligosaccharide sequence NeuAc alpha 2,3Gal present on glycolipids and glycoproteins including CD43, CD162, CD206, or CD227. CD169 serves as an adhesion molecule that can mediate cellular interactions with the extracellular matrix or with other cells including lymphocytes and myeloid cells. CD169 may play a role in erythroid and myeloid cell development as well as leucocyte trafficking and inflammation.
Development References (4)
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Crocker PR, Freeman S, Gordon S, Kelm S. Sialoadhesin binds preferentially to cells of the granulocytic lineage.. J Clin Invest. 1995; 95(2):635-43. (Immunogen: Blocking, Radioimmunoassay). View Reference
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Ducreux J, Crocker PR, Vanbever R. Analysis of sialoadhesin expression on mouse alveolar macrophages.. Immunol Lett. 2009; 124(2):77-80. (Clone-specific: Blocking, Inhibition). View Reference
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Kumamoto Y, Higashi N, Denda-Nagai K, et al. Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type C-type lectin 1.. J Biol Chem. 2004; 279(47):49274-80. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Western blot). View Reference
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Nath D, van der Merwe PA, Kelm S, Bradfield P, Crocker PR. The amino-terminal immunoglobulin-like domain of sialoadhesin contains the sialic acid binding site. Comparison with CD22.. J Biol Chem. 1995; 270(44):26184-91. (Clone-specific: Functional assay, Immunoaffinity chromatography). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.