R718 Mouse Anti-Human CD104 (Integrin β4)

BD Horizon™ R718 Mouse Anti-Human CD104 (Integrin β4)

Name: R718 Mouse Anti-Human CD104 (Integrin β4)
Brand: R718 Mouse Anti-Human CD104 (Integrin β4)
SKU: 570782

Clone ASC-8 (RUO)

Flow cytometric analysis of CD104 (Integrin β4) expression on Human A-431 cells. Cells from the Human A-431 (Human epidermoid carcinoma, ATCC® CRL-1555™) cell line were stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; dashed line histogram) or BD Horizon™ R718 Mouse Anti-Human CD104 (Integrin β4) antibody (Cat. No. 570782/570860; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing CD104 (Integrin β4) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

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Product Details

Brand: BD Horizon™

Alternative Name: ITGB4; CD104; integrin beta-4

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: SCC9 Human Squamous Carcinoma Cells

Application: Flow cytometry (Routinely Tested)

Workshop Number: VI A008

Entrez Gene ID: 3691

RRID: AB_3686038

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

R718 Mouse IgG1, κ Isotype Control RUO

Size 50 µg Cat No. 566928

DAPI Solution RUO

Size 1 mg Cat No. 564907

570782 Rev. 1

Antibody Details

ASC-8

The ASC-8 monoclonal antibody specifically recognizes CD104 which is also known as Integrin beta 4 (Integrin β4 or β4 Integrin). Integrins mediate important intercellular and cell-extracellular matrix interactions which are essential for cellular adhesion and migration as well as for regulating cellular growth and survival. CD104 (Integrin β4) is an ~205 kDa single-pass type I transmembrane glycoprotein that is encoded by ITGB4 (Integrin subunit beta 4). CD104 (Integrin β4) noncovalently associates with CD49f (Integrin α6 chain) to form the Integrin α6/β4 (CD49f/CD104) complex. CD104 (Integrin β4) is expressed on basal epithelial cells, Schwann cells, and some endothelial cells and tumor cells. The CD49f/CD104 complex serves as an adhesion receptor that binds to laminins and keratin filaments. It is a component of epidermal hemidesmosomes that facilitate the stable adhesion of basal epithelial cells to the underlying basement membrane.

570782 Rev. 1

Format Details

R718

The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: R718

Excitation Source: Red 627-640 nm

Excitation Max: 695 nm

Emission Max: 718 nm

570782 Rev.1

Citations & References

View product citations for antibody "570782" on CiteAb

Development References (10)

Egles C, Huet HA, Dogan F, et al. Integrin-blocking antibodies delay keratinocyte re-epithelialization in a human three-dimensional wound healing model.. PLoS One. 2010; 5(5):e10528. (Clone-specific: Flow cytometry, Functional assay). View Reference
Hemler ME, Crouse C, Sonnenberg A. Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines. J Biol Chem. 1989; 264(11):6529-6535. (Biology). View Reference
Jones JC, Kurpakus MA, Cooper HM, Quaranta V. A function for the integrin alpha 6 beta 4 in the hemidesmosome. Cell Regul. 1991; 2(6):427-438. (Biology). View Reference
Kennel SJ, Epler RG, Lankford TK, et al. Second generation monoclonal antibodies to the human integrin alpha 6 beta 4. Hybridoma. 1990; 9(3):243-255. (Biology). View Reference
Mohri T, Adachi Y, Ikehara S, Hioki K, Tokunaga R, Taketani S. Activated Rac1 selectively up-regulates the expression of integrin alpha6beta4 and induces cell adhesion and membrane ruffles of nonadherent colon cancer Colo201 cells.. Exp Cell Res. 1999; 253(2):533-40. (Clone-specific: Flow cytometry, Functional assay). View Reference
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Skubitz AP, Bast RC, Wayner EA, Letourneau PC, Wilke MS. Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.. Am J Pathol. 1996; 148(5):1445-61. (Immunogen: Flow cytometry). View Reference
Sonnenberg A, Calafat J, Janssen H, et al. Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion.. J Cell Biol. 1991; 113(4):907-17. (Biology). View Reference
Tamura RN, Rozzo C, Starr L, et al. Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4.. J Cell Biol. 1990; 111(4):1593-604. (Biology). View Reference
Tanaka Y, Adachi T, Gianocotti F. CD104 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:432-434.

View All (10)

570782 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.