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Purified NA/LE Mouse Anti-Human CD42B
Product Details
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BD Pharmingen™
GP1BA; GPIbalpha; GP-Ib alpha; GPIb-alpha; CD42b-alpha; Glycocalicin; BSS
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested), Functional assay (Reported)
1.0 mg/ml
IV P40
2811
AB_11154402
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
562255 Rev. 1
Antibody Details
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HIP1

The HIP1 monoclonal antibody specifically binds to CD42b. CD42b is also known as the Platelet glycoprotein Ib alpha chain that is encoded by the GP1BA gene. CD42b is disulfide bonded to CD42c to form a 170 kDa heterodimer, GPIb. GPIb forms a noncovalent complex with CD42a and CD42d (CD42 complex) that is expressed on platelets and megakaryocytes. The CD42 complex serves as the von Willebrand Factor  (vWF) surface receptor involved in the adhesion of platelets to the subendothelium of damaged vascular walls. HIP1 inhibits the ristocetin-dependent binding of  vWF to platelets and partially inhibits collagen-induced aggregation.

562255 Rev. 1
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
562255 Rev.1
Citations & References
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Development References (4)

  1. George NP, Wei Q, Shin PK, Konstantopoulos K, Ross JM. Staphylococcus aureus adhesion via Spa, ClfA, and SdrCDE to immobilized platelets demonstrates shear-dependent behavior. Arterioscler Thromb Vasc Biol. 2006; 26(10):2394-2400. (Clone-specific: Functional assay). View Reference
  2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  4. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
View All (4) View Less
562255 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.