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Purified Mouse Anti-Pig SLA-DR
Product Details
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BD Pharmingen™
Pig (QC Testing)
Mouse B10.PD1 IgG2a, κ
Mouse L cells transfected with pig SLA-DR gene
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
AB_394964
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
553642 Rev. 6
Antibody Details
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1053H2-18

The 1053h2-18-1 antibody reacts with swine leukocyte antigen-DR (SLA-DR) expressed on subsets of pig B and T lymphocytes and monocytes, but not on granulocytes. It also reacts with endothelial cells, Langerhans cells, thymocytes, and macrophages. An interesting observation is that SLA-DR is preferentially expressed on CD8+ T cells.

553642 Rev. 6
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553642 Rev.6
Citations & References
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Development References (1)

  1. Lunney JK, Pescovitz MD. Phenotypic and functional characterization of pig lymphocyte populations. Vet Immunol Immunopathol. 1987; 17(1-4):135-144. (Biology). View Reference
553642 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.