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Purified Mouse Anti-p38 MAPK (pT180/pY182)
Purified Mouse Anti-p38 MAPK (pT180/pY182)

Western blot analysis for p38 MAPK (pT180/pY182) (left figure).  HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2) were either left untreated (lane 1) or treated with 25 µg/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37°C (lane 2). The top panel was probed with a mouse anti-p38α antibody (cat.# 612168) and the bottom was probed with the mouse anti-p38 (pT180/pY182) antibody.

Immunofluorescence staining for p38 MAPK (pT180/pY182) (right figure).  HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2) were either untreated (upper left and bottom left panels) or were treated for 15 min with 25 µg/mL anisomycin, an antibiotic and protein synthesis inhibitor (upper right and bottom right panels). Cells were fixed in 3.75% paraformaldehyde and permeabilized with 0.2% Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38α antibody (cat.#612168) (upper left and upper right panels) or with the mouse anti-p38 MAPK (pT180/pY182) antibody (bottom left and bottom right panels).

Western blot analysis for p38 MAPK (pT180/pY182) (left figure).  HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2) were either left untreated (lane 1) or treated with 25 µg/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37°C (lane 2). The top panel was probed with a mouse anti-p38α antibody (cat.# 612168) and the bottom was probed with the mouse anti-p38 (pT180/pY182) antibody.

Immunofluorescence staining for p38 MAPK (pT180/pY182) (right figure).  HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2) were either untreated (upper left and bottom left panels) or were treated for 15 min with 25 µg/mL anisomycin, an antibiotic and protein synthesis inhibitor (upper right and bottom right panels). Cells were fixed in 3.75% paraformaldehyde and permeabilized with 0.2% Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38α antibody (cat.#612168) (upper left and upper right panels) or with the mouse anti-p38 MAPK (pT180/pY182) antibody (bottom left and bottom right panels).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human p38 MAPK (pT180/pY182)
Western blot (Routinely Tested), Flow cytometry, Immunofluorescence (Tested During Development)
38-42 kDa
250 µg/ml
AB_399598
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
612280 Rev. 1
Antibody Details
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30/p38 MAPK (pT180/pY182)

Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytic cells results in the production and release of proinflammatory  cytokines, such as IL-1 and TNF-α. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAP kinases include p38α, β, γ, and δ. These ser/thr kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in muliple signal transduction pathways.

612280 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612280 Rev.1
Citations & References
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Development References (3)

  1. Brunet A, Pouyssegur J. Identification of MAP kinase domains by redirecting stress signals into growth factor responses. Science. 1996; 272(5268):1652-1655. (Biology). View Reference
  2. Han J, Lee JD, Bibbs L, Ulevitch RJ. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science. 1994; 265(5173):808-811. (Biology). View Reference
  3. Winston BW, Chan ED, Johnson GL, Riches DW. Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages. J Immunol. 1997; 159(9):4491-4497. (Biology). View Reference
612280 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.