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Purified Mouse Anti-Human CD1a
Purified Mouse Anti-Human CD1a

Immunohistochemcial staining of dendritic and langerhans cells. Frozen section of human skin was reacted with HI149 antibody. Dendritic cells in the epithelia can be identified by intense brown labeling of their cell surface membranes. Amplification 20X.

Immunohistochemcial staining of dendritic and langerhans cells. Frozen section of human skin was reacted with HI149 antibody. Dendritic cells in the epithelia can be identified by intense brown labeling of their cell surface membranes. Amplification 20X.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
31.25 µg/ml
V 5T CD01.01
909
AB_393637
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: The HI149 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were human skin and tonsil. The antibody stains dendritic cells and Langerhans cells. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, the HI149 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure using the Anti-mouse Ig HRP Detection Kit (Cat. No. 551011). A detailed protocol can be found on our website at www.bdbiosciences.com/support/resources. The clone HI149 is not recommended for formalin-fixed paraffin embedded sections.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550366 Rev. 3
Antibody Details
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HI149

The HI149 monoclonal antibody specifically binds to CD1a. CD1a is a type I transmembrane glycoprotein. The 49 kDa CD1a polypeptide is associated with β2-microglobulin. CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation.

550366 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550366 Rev.3
Citations & References
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Development References (4)

  1. Calabi F, Bradbury A. The CD1 system. Tissue Antigens. 1991; 37(1):1-9. (Biology). View Reference
  2. Hanau D, Schmitt DA, Bieber T, Schmitt D, Cazenave JP. Possible mechanism of action of CD1a antigens. J Invest Dermatol. 1990; 95(5):503-505. (Biology). View Reference
  3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
View All (4) View Less
550366 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.