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PE Rat Anti-Mouse CD84
PE Rat Anti-Mouse CD84
Two-color flow cytometric analysis of CD84 expression on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse NK1.1 antibody (Cat. No. 550627/561117) and either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse CD84 antibody (Cat. No. 566660; Right Plot) at 1 µg/test. Two-color flow cytometric dot plots showing the correlated expression of CD84 (or Ig Isotype Control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD84 expression on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse NK1.1 antibody (Cat. No. 550627/561117) and either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse CD84 antibody (Cat. No. 566660; Right Plot) at 1 µg/test. Two-color flow cytometric dot plots showing the correlated expression of CD84 (or Ig Isotype Control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
Cd84; CDw84; SLAM family member 5; SLAMF5
Mouse (QC Testing)
Rat IgG1, κ
Mouse CD84 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
12523
AB_2869817
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566660 Rev. 1
Antibody Details
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1D3/CD84

The 1D3/CD84 monoclonal antibody specifically recognizes CD84 which is also known as Signaling lymphocytic activation molecule 5 (SLAM family member 5 or Slamf5). CD84 is a single-pass type I transmembrane glycoprotein that is encoded by Cd84 (CD84 antigen) which belongs to the SLAM family within the CD2-subset of the immunoglobulin superfamily (IgSF) of cell surface receptors. CD84 is comprised of an extracellular region with one N-terminal Ig-like V-type (IgV) domain followed by one Ig-like C2-type (IgC2) domain. Its cytoplasmic tail contains two immunoreceptor tyrosine-based switch motifs (ITSMs) and serves as a docking site for activating adaptor molecules, SLAM-associated protein (SAP) or EWS-activated transcript 2 (EAT-2), or inhibitory SH2-binding phosphatases. CD84 is variably expressed on B cells, T cells, NK cells, monocytes, macrophages, dendritic cells, granulocytes, mast cells, hematopoietic progenitor cells, and platelets. CD84 is a homotypic adhesion molecule that can function as a coreceptor in the regulation of innate and adaptive immune responses including the enhancement of T cell proliferation and cytokine production.

        

566660 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566660 Rev.1
Citations & References
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Development References (2)

  1. Cruz-Munoz ME, Dong Z, Shi X, Zhang S, Veillette A. Influence of CRACC, a SLAM family receptor coupled to the adaptor EAT-2, on natural killer cell function.. Nat Immunol. 2009; 10(3):297-305. (Clone-specific: Flow cytometry). View Reference
  2. Guo H, Cruz-Munoz ME, Wu N, Robbins M, Veillette A. Immune cell inhibition by SLAMF7 is mediated by a mechanism requiring src kinases, CD45, and SHIP-1 that is defective in multiple myeloma cells.. Mol Cell Biol. 2015; 35(1):41-51. (Clone-specific: Flow cytometry). View Reference
566660 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.