PE Rat Anti-Mouse CD54 (ICAM-1)

BD Pharmingen™ PE Rat Anti-Mouse CD54 (ICAM-1)

Name: PE Rat Anti-Mouse CD54 (ICAM-1)
Brand: BD Pharmingen™
SKU: 568533

Clone YN1/1.7.4 (also known as YN1/1.7)

(RUO)

Flow cytometric analysis of CD54 (ICAM-1) expression on Resting or Activated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were either not activated (Resting; Left Panel) or were stimulated with lipopolysaccharide (LPS) for 2 days (Activated; Right Panel). The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and then stained with either PE Rat IgG2b, κ Isotype Control (Cat No. 555848; dashed line histograms) or PE Rat Anti-Mouse CD54 (ICAM-1) antibody (Cat No. 568533/568539; solid line histograms) at 0.125 µg/test. The fluorescence histograms showing CD54 (ICAM-1) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD Fortessa™ X-20 Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Icam-1; Icam1; intercellular adhesion molecule 1; Ly-47; MALA-2; myD10

Reactivity: Mouse (QC Testing)

Isotype: Rat F344, also known as Fischer, CDF IgG2b, κ

Immunogen: Mouse NS-1 Cells

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Rat IgG2b, κ Isotype Control RUO

Size 100 Tests Cat No. 555848

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

Lysing Buffer RUO

Size 100 mL Cat No. 555899

568533 Rev. 1

Antibody Details

YN1/1.7.4

The YN1/1.7.4 monoclonal antibody specifically binds to CD54 which is also known as Intercellular adhesion molecule 1 (ICAM-1). CD54 (ICAM-1) is a ~90-110 kDa single-pass type I transmembrane glycoprotein that is encoded by Icam1 which belongs to the Immunoglobulin gene superfamily (IgSF). This adhesion molecule is comprised of five IgC2-like domains in its extracellular region, a transmembrane segment, and a cytoplasmic tail. It is expressed on T and B lymphocytes, monocytes, macrophages, dendritic cells (DC), endothelial cells, and epithelial cells. Through binding to cell surface ligands including LFA1 (CD11a/CD18) and Mac-1 (CD11b/CD18), CD54 (ICAM-1) serves to mediate intercellular connections between T cells, B cells, endothelial cells, antigen-presenting cells, and target cells. Its expression is upregulated upon stimulation by inflammatory mediators including proinflammatory cytokines such as IL-1, IFN-γ, and TNF as well as lipopolysaccharide (LPS). CD54 (ICAM-1) participates in leucocyte trans-endothelial migration and trafficking, inflammatory reactions, and antigen-specific immune responses. The YN1/1.7.4 antibody reportedly blocks in vitro and in vivo intercellular adhesion functions mediated by CD54 (ICAM-1) involved in inflammation and immune responses.

568533 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

568533 Rev.1

Citations & References

View product citations for antibody "568533" on CiteAb

Development References (3)

Horley KJ, Carpenito C, Baker B, Takei F. Molecular cloning of murine intercellular adhesion molecule (ICAM-1).. EMBO J. 1989; 8(10):2889-96. (Clone-specific: Blocking, Flow cytometry, Functional assay, Immunoaffinity chromatography, Inhibition). View Reference
Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2).. J Immunol. 1985; 134(3):1403-7. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, Radioimmunoassay). View Reference
Wang Q, Zhang M, Ding G, et al. Anti-ICAM-1 antibody and CTLA-4Ig synergistically enhance immature dendritic cells to induce donor-specific immune tolerance in vivo.. Immunol Lett. 2003; 90(1):33-42. (Clone-specific: In vivo exacerbation). View Reference

568533 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.