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PE Rat Anti-Human Folate Receptors α/β (FRαβ)

PE Rat Anti-Human Folate Receptors α/β (FRαβ)

Clone 5 (also known as No.5; No. 5; Antibody No.5; Antibody No. 5; Clone 5; 5/FRAB)

(RUO)
PE Rat Anti-Human Folate Receptors α/β (FRαβ)

Two-parameter flow cytometric analysis of Folate Receptors α/β (FRαβ) expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Human Human Folate Receptors α/β (FRαβ) antibody (Cat. No. 567605; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A bivariate pseudocolor density plot showing the correlated expression of Folate Receptors α/β (FRαβ) [or Ig Isotype control staining] versus side-light scatter (SSC-A) signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

PE Rat Anti-Human Folate Receptors α/β (FRαβ)

Flow cytometric analysis of Folate Receptors α/β (FRαβ) expression on HeLa Cells. Cells from the human HeLa (Cervical adenocarcinoma, ATCC CCL-2) cell line were stained with either PE Rat IgG2a, κ Isotype Control (dotted line histogram) or PE Rat Anti-Human Folate Receptors α,β antibody (Cat. No. 567605; solid line histogram) at 1 µg/test. The fluorescence histograms were derived from gated events with the light-scatter characteristics of viable HeLa cells. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.

Two-parameter flow cytometric analysis of Folate Receptors α/β (FRαβ) expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Human Human Folate Receptors α/β (FRαβ) antibody (Cat. No. 567605; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A bivariate pseudocolor density plot showing the correlated expression of Folate Receptors α/β (FRαβ) [or Ig Isotype control staining] versus side-light scatter (SSC-A) signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

Flow cytometric analysis of Folate Receptors α/β (FRαβ) expression on HeLa Cells. Cells from the human HeLa (Cervical adenocarcinoma, ATCC CCL-2) cell line were stained with either PE Rat IgG2a, κ Isotype Control (dotted line histogram) or PE Rat Anti-Human Folate Receptors α,β antibody (Cat. No. 567605; solid line histogram) at 1 µg/test. The fluorescence histograms were derived from gated events with the light-scatter characteristics of viable HeLa cells. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.

Product Details
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BD Pharmingen™
FOLR1, Folate receptor 1, FR-alpha, FRalpha; FOLR2, Folate receptor 2, FR-beta, FRbeta; FRαβ, FR-αβ
Human (QC Testing)
Rat Wistar IgG2a, κ
Human Folate Receptor beta Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
567605 Rev. 2
Antibody Details
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5

Clone 5 (aka, 5/FRAB) is a monoclonal antibody that specifically recognizes the human Folate Receptor alpha (FRα) and Folate Receptor beta (FRβ), ie, Folate Receptors α/β (FRαβ or FR-αβ). FRα and FRβ are glycosylphosphatidylinositol (GPI)-linked membrane proteins that are encoded by FOLR1 and FOLR2 which belong to the folate-binding receptor family, respectively. These cell surface receptors share ~70% amino acid sequence homology and bind folate with high affinity. The receptors transport folate into cells which is essential for methionine and DNA synthesis that support cell growth and proliferation. FRα is primarily expressed on epithelial cells in normal tissues, eg, the choroid plexus and proximal kidney tubule, and on some tumor cells of epithelial origin, eg, in some ovarian cancers. FRβ is expressed on placental cells and on monocytes as well as macrophages found in normal and diseased tissues, eg, in rheumatoid arthritis. Clone 5 can reportedly mediate Complement-dependent cytotoxicity (CDC) and Antibody-dependent cellular phagocytosis (ADCP) activity. Soluble forms of FRα and FRβ have also been described which can be found in various body fluids such as plasma or milk.

567605 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
567605 Rev.2
Citations & References
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Development References (4)

  1. Holm J, Hansen SI. Characterization of soluble folate receptors (folate binding proteins) in humans. Biological roles and clinical potentials in infection and malignancy. Biochim Biophys Acta Proteins Proteom. 2020; 1868(10):1-10. (Biology). View Reference
  2. McNulty H, Pentieva K, Hoey L, Strain J, Ward M. Nutrition throughout life: folate.. Int J Vitam Nutr Res. 2012; 82(5):348-54. (Biology). View Reference
  3. Nagai T, Furusho Y, Li H, et al. Production of a High-affinity Monoclonal Antibody Reactive with Folate Receptors Alpha and Beta.. Monoclon Antib Immunodiagn Immunother. 2015; 34(3):181-90. (Immunogen: Cytotoxicity, Flow cytometry, Functional assay). View Reference
  4. Otsubo H, Tsuneyoshi Y, Nakamura T, Matsuda T, Komiya S, Matsuyama T. Serum-soluble folate receptor β as a biomarker for the activity of rheumatoid arthritis synovitis and the response to anti-TNF agents.. Clin Rheumatol. 2018; 37(11):2939-2945. (Clone-specific: ELISA). View Reference
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567605 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.