PE Mouse Anti-Mouse I-E[k]

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [753608] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ PE Mouse Anti-Mouse I-E[k]

Name: PE Mouse Anti-Mouse I-E[k]
Brand: BD Pharmingen™
SKU: 553544

Clone 14-4-4S

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing), Rat (Reported)

Isotype: Mouse C3H.SW IgG2a, κ

Immunogen: C3H mouse skin graft and splenocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_394911

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

PE Mouse IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 553457

553544 Rev. 10

Antibody Details

14-4-4S

The 14-4-4S monoclonal antibody specifically recognizes the I-E[k] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[r] haplotypes. Cells from mice of the H-2[b], H-2[f], H-2[g7], H-2[q], and H-2[s] haplotypes do not express I-E antigen. It has been reported that mAb 14-4-4S crossreacts with the rat MHC class II antigen RT1D.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553544 Rev. 10

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

553544 Rev.10

Citations & References

Development References (5)

Blankenhorn EP, Symington FW, Cramer DV. Biochemical characterization of Ia antigens encoded by the RT1.B and RT1.D loci in the rat MHC. Immunogenetics. 1983; 17(5):475-484. (Clone-specific). View Reference
Hattori M, Buse JB, Jackson RA, et al. The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex. Science. 1986; 231(4739):733-735. (Biology). View Reference
Klein J. Mutations in H-2E loci. In: Klein J. Natural History of the Major Histocompatibility Complex. New York: John Wiley & Sons; 1986:216-218.
Neiss U, Reske K. Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture. Int Immunol. 1994; 6(1):61-71. (Clone-specific). View Reference
Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980; 124(2):533-540. (Immunogen). View Reference

View All (5)

553544 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.