Skip to main content Skip to navigation
PE Mouse Anti-Human Galectin-3
PE Mouse Anti-Human Galectin-3
Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Galectin-3 antibody (Cat. No. 565676; solid line histogram) at 0.5 ug/test. The fluorescence histogram showing Galectin-3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Galectin-3 antibody (Cat. No. 565676; solid line histogram) at 0.5 ug/test. The fluorescence histogram showing Galectin-3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data on this Technical Data Sheet are not lot specific.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
LGALS3; Gal-3; GAL3; GALIG; GALBP; CBP35; MAC-2; IgE-binding protein; L31
Human (QC Testing), Mouse (Reported)
Mouse BALB/c IgG1, κ
Recombinant Human Galectin-3
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869698
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
565676 Rev. 1
Antibody Details
Down Arrow Up Arrow
B2C10

The B2C10 monoclonal antibody specifically recognizes Galectin-3 (GAL3/Gal-3) which is also known as, IgE-binding protein (εBP/IgEBP), Carbohydrate-binding protein 35 (CBP35), Galactoside-binding protein (GALBP), or  MAC-2. Galectin-3 is a 30 kDa protein that is encoded by LGALS3 (lectin, galactoside-binding, soluble, 3) and belongs to the galactoside-binding lectins family. It is expressed by a variety of cell types including monocytes/macrophages, dendritic cells, some activated lymphocytes, fibroblasts, epithelial cells, and tumor cells. Galectin-3 has diverse functions and is involved in the induction and regulation of immune responses, inflammation, cell adhesion, chemotaxis, apoptosis, angiogenesis, and fibrosis. Galectin-3 can be differentially expressed in the nucleus, cytoplasm, and surface of cells and can be secreted. Galectin-3 levels can be markedly elevated in the nuclei of proliferating cells suggesting that it may play a role in cell growth regulation. Nuclear galectin-3 has been associated with gene expression and pre-mRNA splicing. Cell surface Galectin-3 reportedly serves as a non-integrin, laminin-binding protein, suggesting its role in cell adhesion to basement membranes. The B2C10 antibody is suitable for immunohistochemical staining of paraffin-embedded tissue sections. The B2C10 antibody reportedly crossreacts with mouse Galectin-3.

565676 Rev. 1
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565676 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (9)

  1. Acosta-Rodríguez EV, Montes CL, Motrán CC, et al. Galectin-3 mediates IL-4-induced survival and differentiation of B cells: functional cross-talk and implications during Trypanosoma cruzi infection.. J Immunol. 2004; 172(1):493-502. (Clone-specific: Flow cytometry). View Reference
  2. Cherayil BJ, Weiner SJ, Pillai S. The Mac-2 antigen is a galactose-specific lectin that binds IgE. J Exp Med. 1989; 170(6):1959-1972. (Biology). View Reference
  3. Cortegano I, del Pozo V, Cárdaba B, et al. Galectin-3 down-regulates IL-5 gene expression on different cell types. J Immunol. 1998; 161(1):385-389. (Biology). View Reference
  4. Haudek KC, Spronk KJ, Voss PG, Patterson RJ, Wang JL, Arnoys EJ. Dynamics of galectin-3 in the nucleus and cytoplasm.. Biochim Biophys Acta. 2010; 1800(2):181-9. (Biology). View Reference
  5. Liu FT, Hsu DK, Zuberi RI, et al. Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains.. Biochemistry. 1996; 35(19):6073-9. (Immunogen: Bioassay, Blocking, ELISA, Functional assay, Inhibition, Radioimmunoassay, Western blot). View Reference
  6. Mey A, Leffler H, Hmama Z, Normier G, Revillard JP. The animal lectin galectin-3 interacts with bacterial lipopolysaccharides via two independent sites. J Immunol. 1996; 156(4):1572-1577. (Biology). View Reference
  7. Newlaczyl AU, Yu LG. Galectin-3--a jack-of-all-trades in cancer.. Cancer Lett. 2011; 313(2):123-8. (Biology). View Reference
  8. Ocklenburg F, Moharregh-Khiabani D, Geffers R, et al. UBD, a downstream element of FOXP3, allows the identification of LGALS3, a new marker of human regulatory T cells.. Lab Invest. 2006; 86(7):724-37. (Clone-specific: Flow cytometry). View Reference
  9. Sano H, Hsu DK, Yu L, et al. Human galectin-3 is a novel chemoattractant for monocytes and macrophages.. J Immunol. 2000; 165(4):2156-64. (Clone-specific: Bioassay, Functional assay, Inhibition). View Reference
View All (9) View Less
565676 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.