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PE Mouse Anti-Human CD101
PE Mouse Anti-Human CD101

Multiparameter flow cytometric analysis of CD101 expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human CD101 antibody (Cat. No. 566371; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Contour plots showing the correlated expression of CD101 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.

PE Mouse Anti-Human CD101

Two-color flow cytometric analysis of CD101 expression on human peripheral blood lymphocytes. Whole blood cells were stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, Left Plot) or PE Mouse Anti-Human CD101 antibody (Cat. No. 566371; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of CD3 versus CD101 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.

Multiparameter flow cytometric analysis of CD101 expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human CD101 antibody (Cat. No. 566371; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Contour plots showing the correlated expression of CD101 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.

Two-color flow cytometric analysis of CD101 expression on human peripheral blood lymphocytes. Whole blood cells were stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, Left Plot) or PE Mouse Anti-Human CD101 antibody (Cat. No. 566371; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of CD3 versus CD101 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.

Product Details
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BD Pharmingen™
IGSF2; Cell surface glycoprotein V7; V7; EWI-101; P126
Human (QC Testing)
Mouse BALB/c IgG1, κ
T cell clone CS1
Flow cytometry (Routinely Tested)
5 µl
VI M12
9398
AB_2739714
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566371 Rev. 1
Antibody Details
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V7.1

The V7.1 monoclonal antibody specifically recognizes CD101 which is also known as P126 or Glu-Trp-Ile EWI motif-containing protein 101 (EWI-101). CD101 is encoded by IGSF2 (Immunoglobulin superfamily member 2) and belongs to the EWI (sharing a conserved glutamine-tryptophan-isoleucine motif) family within the Ig superfamily. CD101 is a ~135-140 kDa type I transmembrane glycoprotein that has a short cytoplasmic domain with potential phosphorylation sites and an extracellular region with seven immunoglobulin-variable (IgV)-like domains. CD101 is expressed as homodimers by granulocytes, monocytes, dendritic cells, and some T cells. Its expression by T cells may be upregulated upon activation through the CD3 complex. CD101 may play a role in TCR/CD3-dependent T-cell activation.

566371 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566371 Rev.1
Citations & References
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Development References (4)

  1. Boumsell L, Hall KT, Freeman GJ, Benussan A. CD101 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:1033-1034.
  2. Rivas A, Ruegg CL, Zeitung J, et al. V7, a novel leukocyte surface protein that participates in T cell activation. I. Tissue distribution and functional studies.. J Immunol. 1995; 154(9):4423-33. (Immunogen). View Reference
  3. Ruegg CL, Rivas A, Madani ND, Zeitung J, Laus R, Engleman EG. V7, a novel leukocyte surface protein that participates in T cell activation. II. Molecular cloning and characterization of the V7 gene.. J Immunol. 1995; 154(9):4434-43. (Clone-specific: Immunofluorescence, Immunoprecipitation, Radioimmunoassay). View Reference
  4. Soares LR, Rivas A, Ruegg C, Engleman EG. Differential response of CD4+ V7+ and CD4+ V7- T cells to T cell receptor-dependent signals: CD4+ V7+ T cells are co-stimulation independent and anti-V7 antibody blocks the induction of anergy by bacterial superantigen.. Eur J Immunol. 1997; 27(6):1413-21. (Clone-specific: Inhibition). View Reference
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566371 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.