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FITC Rat Anti-Mouse CD71

FITC Rat Anti-Mouse CD71

Clone RI7217 (also known as RI7 217.1.3; R17 217.1.4; R17 217; R17217)

(RUO)
FITC Rat Anti-Mouse CD71

Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat anti-Mouse TER-119/Erythroid Cells antibody (Cat. No. 561033/ 557909) and either FITC Rat IgG2a, κ Isotype Control (Cat. No. 557228; Left Plot) or FITC Rat Anti-Mouse CD71 antibody (Cat. No. 567260; Right Plot) at 0.5 μg/test. 7-AAD (7-Amino-Actinomycin D) Solution (Cat. No. 559925) was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression (or Ig Isotype control staining) versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD Fortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat anti-Mouse TER-119/Erythroid Cells antibody (Cat. No. 561033/ 557909) and either FITC Rat IgG2a, κ Isotype Control (Cat. No. 557228; Left Plot) or FITC Rat Anti-Mouse CD71 antibody (Cat. No. 567260; Right Plot) at 0.5 μg/test. 7-AAD (7-Amino-Actinomycin D) Solution (Cat. No. 559925) was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression (or Ig Isotype control staining) versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD Fortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
Tfrc; TFR; TFR1; TR; Trfr; Mtvr1
Mouse (QC Testing)
Rat BDIX IgG2a, κ
Mouse erythroleukemia
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
567260 Rev. 1
Antibody Details
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RI7217

The RI7217 monoclonal antibody specifically recognizes the mouse Transferrin Receptor (TfR, Trfr, or TR), which is also known as Transferrin Receptor protein 1 (TfR1), CD71, and Mammary tumor virus receptor 1 (Mtvr-1 or Mtvr1). CD71 is a ~95-kDa single-pass type II transmembrane glycoprotein that is encoded by Tfrc and expressed on the cell surface as a disulfide-linked homodimer. The CD71 monomer is comprised of an extracellular C-terminal domain with a transferrin binding site followed by a transmembrane region and an N-terminal cytoplasmic tail that is involved in mediating the endocytosis and recycling of the receptor. This receptor mediates cellular iron uptake via endocytosis of an iron-transferrin complex followed by the recycling of transferrin and the receptor to the cell surface. CD71 is lowly expressed on most nonactivated cells including resting B and T lymphocytes. CD71 expression is upregulated on activated and proliferating cells such as mitogen- or antigen-stimulated B cells and T cells as well as some tumor cells. CD71 is variably expressed on erythroid precursors from pronormoblasts (proerythroblasts) to reticulocytes and is absent on mature red blood cells. CD71 is expressed on erythroid precursors that utilize iron for heme synthesis. CD71 can also serve as an entry receptor for several viral infections including those caused by Mouse mammary tumor virus (MMTV). The RI7217 antibody can reportedly be used to inhibit cellular proliferation.

567260 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
567260 Rev.1
Citations & References
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Development References (6)

  1. Ernst DN, McQuitty DN, Weigle WO, Hobbs MV. Expression of membrane activation antigens on murine B lymphocytes stimulated with lipopolysaccharide. Cell Immunol. 1988; 114(1):161-173. (Clone-specific: Flow cytometry). View Reference
  2. Ernst DN, Weigle WO, McQuitty DN, Rothermel AL, Hobbs MV. Stimulation of murine T cell subsets with anti-CD3 antibody. Age-related defects in the expression of early activation molecules. J Immunol. 1989; 142(5):1413-1421. (Clone-specific: Flow cytometry). View Reference
  3. Kemp JD, Thorson JA, Gomez F, Smith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989; 122(1):218-230. (Clone-specific: Functional assay, Inhibition). View Reference
  4. Lesley J, Hyman R, Schulte R, Trotter J. Expression of transferrin receptor on murine hematopoietic progenitors.. Cell Immunol. 1984; 83(1):14-25. (Immunogen: Flow cytometry). View Reference
  5. Lesley JF, Schulte RJ. Inhibition of cell growth by monoclonal anti-transferrin receptor antibodies.. Mol Cell Biol. 1985; 5(8):1814-21. (Clone-specific: Blocking, Flow cytometry, Functional assay, Inhibition). View Reference
  6. Motamedi M, Xu L, Elahi S. Correlation of transferrin receptor (CD71) with Ki67 expression on stimulated human and mouse T cells: The kinetics of expression of T cell activation markers.. J Immunol Methods. 2016; 437:43-52. (Biology: Flow cytometry). View Reference
View All (6) View Less
567260 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.