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BV421 Mouse Anti-Human CD200 Receptor
BV421 Mouse Anti-Human CD200 Receptor

Flow cytometric analysis of OX2R (CD200R1) expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438, Left Plot) or BD Horizon BV421 Mouse Anti-Human OX2R (CD200R1) antibody (Cat. No. 566344; Right Plot) at 1 μg/test. The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The contour plots showing the expression of CD200R (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.

Flow cytometric analysis of OX2R (CD200R1) expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438, Left Plot) or BD Horizon BV421 Mouse Anti-Human OX2R (CD200R1) antibody (Cat. No. 566344; Right Plot) at 1 μg/test. The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The contour plots showing the expression of CD200R (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Horizon™
CD200R1; CD200 receptor 1; hCD200R; CRTR2; HCRTR2; MOX2R; OX2R
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CD200R Recombinant Protein
Flow cytometry/immunoassay (Routinely Tested)
0.2 mg/ml
AB_2739700
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566344 Rev. 1
Antibody Details
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OX-108

The OX-108 monoclonal antibody specifically binds to CD200R which is also known as OX2 receptor 1 (OX2R) or MOX2 receptor (MOX2R). CD200R is a type I transmembrane glycoprotein that is encoded by CD200R1 (CD200 receptor 1) and belongs to the C2-set domain containing gene family within the Ig superfamily. CD200R functions as an inhibitory receptor when bound by its ligand, CD200. CD200R is expressed by hematopoietic cells including monocytes, macrophages, dendritic cells, Langerhans cells, neutrophils, basophils, most T cells and mast cells. CD200R signaling may regulate the functions of various types of leucocytes.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

566344 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566344 Rev.1
Citations & References
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Development References (5)

  1. Caserta S, Nausch N, Sawtell A, et al. Chronic infection drives expression of the inhibitory receptor CD200R, and its ligand CD200, by mouse and human CD4 T cells.. PLoS ONE. 2012; 7(4):e35466. (Biology). View Reference
  2. Jenmalm MC, Cherwinski H, Bowman EP, Phillips JH, Sedgwick JD. Regulation of myeloid cell function through the CD200 receptor.. J Immunol. 2006; 176(1):191-9. (Biology). View Reference
  3. Klatka J, Grywalska E, Klatka M, Rahnama M, Polak A, Rolinski J. Expression of CD200 and CD200R regulatory molecules on the CD83+ monocyte-derived dendritic cells generated from patients with laryngeal cancer.. Folia Histochem Cytobiol. 2013; 51(1):59-65. (Biology). View Reference
  4. Mihrshahi R, Barclay AN, Brown MH. Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cells.. J Immunol. 2009; 183(8):4879-86. (Clone-specific: Cell separation, Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
  5. Wright GJ, Cherwinski H, Foster-Cuevas M, et al. Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.. J Immunol. 2003; 171(6):3034-46. (Immunogen: Flow cytometry). View Reference
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566344 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.