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BUV661 Mouse Anti-Human CD10
Product Details
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BD OptiBuild™
Atriopeptidase; CALLA; Enkephalinase; EPN;Neutral endopeptidase; Neprilysin
Human (Tested in Development)
Mouse IgG1, κ
NALM-6 Pre–B-cell Line
Flow cytometry (Qualified)
0.2 mg/ml
IV B-506; V B-CD10.4
4311
AB_2874391
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV661 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 661 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
750189 Rev. 4
Antibody Details
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MEM-78

The MEM-78 monoclonal antibody specifically recognizes CD10. CD10 is a 100 kDa type II transmembrane, glycosylated, zinc-containing metalloprotease. The CD10 antigen is also known as common acute lymphoblastic leukemia antigen (CALLA), neutral endopeptidase (NEP), gp100, and enkephalinase. The CD10 antigen is found on lymphocytes from samples with acute B-lymphoid leukemia. The CD10 antigen is also present on a wide variety of normal and neoplastic cell types including renal epithelia, fibroblasts, granulocytes, germinal center B lymphocytes, neutrophils, some T-cell leukemias, and some lymphoma, melanoma, and glioma cell lines. The CD10 antigen cleaves a number of biologically active peptides, including fMLP, and may modulate the chemotactic activity of fMLP towards neutrophils. Inhibition of the CD10 antigen promotes B-cell maturation, suggesting that it plays a role in B-cell development.

The antibody was conjugated to BD Horizon™ BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP.  Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).

    

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV661 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.

750189 Rev. 4
Format Details
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BUV661
The BD Horizon Brilliant™ Ultraviolet 661 (BUV661) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 660-nm. BUV661, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 660-nm (e.g., 670/25 bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV661
Ultraviolet 355 nm
350 nm
660 nm
750189 Rev.4
Citations & References
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Development References (14)

  1. Caligaris-Cappio F, Riva M, Tesio L, Schena M, Gaidano G, Bergui L. Human normal CD5+ B lymphocytes can be induced to differentiate to CD5- B lymphocytes with germinal center cell features.. Blood. 1989; 73(5):1259-63. (Biology). View Reference
  2. Connelly JC, Chambless R, Holiday D, Chittenden K, Johnson AR. Up-regulation of neutral endopeptidase (CALLA) in human neutrophils by granulocyte-macrophage colony-stimulating factor.. J Leukoc Biol. 1993; 53(6):685-90. (Biology). View Reference
  3. Connelly JC, Skidgel RA, Schulz WW, Johnson AR, Erdös EG. Neutral endopeptidase 24.11 in human neutrophils: cleavage of chemotactic peptide.. Proc Natl Acad Sci USA. 1985; 82(24):8737-41. (Biology). View Reference
  4. Consolini R, Legitimo A, Rondelli R, et al. Clinical relevance of CD10 expression in childhood ALL. The Italian Association for Pediatric Hematology and Oncology (AIEOP).. Haematologica. 1998; 83(11):967-73. (Biology). View Reference
  5. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD10. In: :33-34. View Reference
  6. Erdös EG, Skidgel RA. Neutral endopeptidase 24.11 (enkephalinase) and related regulators of peptide hormones.. FASEB J. 1989; 3(2):145-51. (Biology). View Reference
  7. Erdös EG, Wagner B, Harbury CB, Painter RG, Skidgel RA, Fa XG. Down-regulation and inactivation of neutral endopeptidase 24.11 (enkephalinase) in human neutrophils.. J Biol Chem. 1989; 264(24):14519-23. (Biology). View Reference
  8. Hofman P, Selva E, Le Negrate G, et al. CD10 inhibitors increase f-Met-Leu-Phe-induced neutrophil transmigration.. J Leukoc Biol. 1998; 63(3):312-20. (Biology). View Reference
  9. Horejsi V, Angelisova P, Bazil V, et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biologica. 1988; 34(1):23-34. (Clone-specific).
  10. LeBien TW, McCormack RT. The common acute lymphoblastic leukemia antigen (CD10)--emancipation from a functional enigma.. Blood. 1989; 73(3):625-35. (Biology). View Reference
  11. Letarte M, Vera S, Tran R, et al. Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase. J Exp Med. 1988; 168(4):1247-1253. (Biology). View Reference
  12. Salles G, Rodewald HR, Chin BS, Reinherz EL, Shipp MA. Inhibition of CD10/neutral endopeptidase 24.11 promotes B-cell reconstitution and maturation in vivo.. Proc Natl Acad Sci USA. 1993; 90(16):7618-22. (Biology). View Reference
  13. Zola H. CD10 Workshop Panel report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:505-507.
  14. Zola H. CD10. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:55.
View All (14) View Less
750189 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.