Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
New Zealand (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Brilliant Stain Buffer Plus

      BD Horizon™ Brilliant Stain Buffer Plus

      (RUO)
      Brilliant Stain Buffer Plus
      Figure 1. Either BD Horizon Brilliant Stain Buffer or BD Horizon Brilliant Stain Buffer Plus should be used in multicolor immunofluorescent staining and flow cytometric analysis when using two or more BD Horizon Brilliant reagents. Whole human blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD4 and BD Horizon™ BV711 Mouse Anti-Human CD19 (Top Plots) antibodies or BD Horizon™ BUV395 Mouse Anti-Human CD4 and BD Horizon™ BV605 Mouse Anti-Human CD19 (Bottom Plots) antibodies. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-color flow cytometric contour plots showing the correlated expression of CD4 versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Staining cells in the presence of BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349; Middle Plots) or BD Horizon Brilliant Stain Buffer Plus (Cat. No. 563795/568264; Right Plots) restores the major lymphocyte populations to their expected fluorescent staining patterns (dotted lines) when compared with cells stained without a Brilliant Stain Buffer (Left Plots). Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      Brilliant Stain Buffer Plus Brilliant Stain Buffer Plus
      Figure 1. Either BD Horizon Brilliant Stain Buffer or BD Horizon Brilliant Stain Buffer Plus should be used in multicolor immunofluorescent staining and flow cytometric analysis when using two or more BD Horizon Brilliant reagents. Whole human blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD4 and BD Horizon™ BV711 Mouse Anti-Human CD19 (Top Plots) antibodies or BD Horizon™ BUV395 Mouse Anti-Human CD4 and BD Horizon™ BV605 Mouse Anti-Human CD19 (Bottom Plots) antibodies. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-color flow cytometric contour plots showing the correlated expression of CD4 versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Staining cells in the presence of BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349; Middle Plots) or BD Horizon Brilliant Stain Buffer Plus (Cat. No. 563795/568264; Right Plots) restores the major lymphocyte populations to their expected fluorescent staining patterns (dotted lines) when compared with cells stained without a Brilliant Stain Buffer (Left Plots). Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      Product Details
      Down Arrow Up Arrow


      BD Horizon™
      Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
      RUO
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.


      Description

      The BD Horizon Brilliant Stain Buffer Plus is a buffer formulated for the immunofluorescent staining of cells. This buffer was developed to have a reduced test volume for applications where total staining volume is a concern. Brilliant Stain Buffer Plus is as effective as BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) in mitigating staining artifacts seen when using multiple BD Horizon Brilliant reagents. For optimal and reproducible results, either BD Horizon Brilliant Stain Buffer Plus or BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant™ dye-conjugated reagents are used. The BD Horizon Brilliant Stain Buffer Plus is compatible with the use of other fluorescent staining reagents conjugated with traditional fluorochromes, such as fluorescein, phycoerythrin or Alexa Fluor® dyes

      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      4. The BD Horizon Brilliant™ Violet, Blue and Ultraviolet dyes and BD Horizon Brilliant Stain Buffer are covered by one or more of the following US patents: 8,110,673; 8,354,239; 8,158,444; 8,227,187; 8,362,193; 8,455,613; 8,575,303; 8,802,450.
      5. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      Show More blue down arrow Show Less blue up arrow
      568264 Rev. 1
      Citations & References
      Down Arrow Up Arrow

      Development References (7)

      1. Acosta JR, Douagi I, Andersson DP, et al. Increased fat cell size: a major phenotype of subcutaneous white adipose tissue in non-obese individuals with type 2 diabetes.. Diabetologia. 2016; 59(3):560-70. (Methodology: Flow cytometry). View Reference
      2. Andrés-Blasco I, Herrero-Cervera A, Vinué Á, et al. Hepatic lipase deficiency produces glucose intolerance, inflammation and hepatic steatosis.. J Endocrinol. 2015; 227(3):179-91. (Methodology: Flow cytometry). View Reference
      3. Ingelman-Sundberg HM, Laestadius Å, Chrapkowska C, et al. Diverse effects on vaccine-specific serum IgG titres and memory B cells upon methotrexate and anti-TNF-α therapy in children with rheumatic diseases: A cross-sectional study.. Vaccine. 2016; 34(10):1304-11. (Methodology: Flow cytometry). View Reference
      4. Leijten EF, van Kempen TS, Boes M, et al. Brief report: enrichment of activated group 3 innate lymphoid cells in psoriatic arthritis synovial fluid.. 2015; 67(10):2673-8. (Methodology: Flow cytometry). View Reference
      5. McKay FC, Gatt PN, Fewings N, et al. The low EOMES/TBX21 molecular phenotype in multiple sclerosis reflects CD56+ cell dysregulation and is affected by immunomodulatory therapies.. Clin Immunol. 2016; 163:96-107. (Methodology: Flow cytometry). View Reference
      6. O'Connor KS, Read SA, Wang M, et al. IFNL3/4 genotype is associated with altered immune cell populations in peripheral blood in chronic hepatitis C infection.. Genes Immun. 2016; 17(6):328-34. (Methodology: Flow cytometry). View Reference
      7. Vinué Á, Andrés-Blasco I, Herrero-Cervera A, et al. Ink4/Arf locus restores glucose tolerance and insulin sensitivity by reducing hepatic steatosis and inflammation in mice with impaired IRS2-dependent signalling.. Biochim Biophys Acta. 2015; 1852(9):1729-42. (Methodology: Flow cytometry). View Reference
      View All (7) View Less
      568264 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.