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      Alexa Fluor™ 647 Rat Anti-Mouse CD98
      Alexa Fluor™ 647 Rat Anti-Mouse CD98
      Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh (Unstimulated; Left Panel) or 48 hours after Concanavalin A (Con A) mitogenic stimulation (Stimulated; Right Panel). The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142] and stained with either Alexa Fluor™ 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dotted line histograms) or Alexa Fluor™ 647 Rat Anti-Mouse CD98 antibody (Cat No. 568193/568194; solid line histograms) at 0.5 µg/test. The fluorescence histograms showing CD98 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Alexa Fluor™ 647 Rat Anti-Mouse CD98
      Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh (Unstimulated; Left Panel) or 48 hours after Concanavalin A (Con A) mitogenic stimulation (Stimulated; Right Panel). The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142] and stained with either Alexa Fluor™ 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dotted line histograms) or Alexa Fluor™ 647 Rat Anti-Mouse CD98 antibody (Cat No. 568193/568194; solid line histograms) at 0.5 µg/test. The fluorescence histograms showing CD98 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      Slc3a2; Cd98; 4F2; 4F2HC; CD98 heavy chain; Ly-10; Ly-m10; Ly10
      Mouse (QC Testing)
      Rat OFA, also known as Outbred OFA IgG2a, κ
      EL4-6.1 Thymoma Cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      8. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      9. An isotype control should be used at the same concentration as the antibody of interest.
      10. For U.S. patents that may apply, see bd.com/patents.
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      568194 Rev. 2
      Antibody Details
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      RL388

      The RL388 monoclonal antibody recognizes CD98 which is also known as the 4F2 antigen and Ly-10. CD98 is a disulfide-linked heterodimer comprised of an ~86 kDa single-pass type II heavy chain glycoprotein that associates with a ~39-kDa multi-pass, nonglycosylated light chain which belongs to the L-type amino transporter family. The RL388 antibody specifically binds to an epitope present on the CD98 heavy chain (CD98hc, also known as the 4F2 cell-surface antigen heavy chain or 4F2hc) which is encoded by Slc3a2 [solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2]. CD98 is variably expressed on different cell types including T cells, B cells, and NK cells as well as monocytes, granulocytes, platelets, and fibroblasts. CD98 serves as an activation marker for T and B cells since its expression is upregulated upon cellular activation. CD98 regulates cellular growth, proliferation, and survival through CD98hc interactions with β integrins and by CD98 light chain-mediated amino acid transport.

      568194 Rev. 2
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      568194 Rev.2
      Citations & References
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      View product citations for antibody "568194" on CiteAb

      Development References (7)

      1. Cantor J, Browne CD, Ruppert R, et al. CD98hc facilitates B cell proliferation and adaptive humoral immunity.. Nat Immunol. 2009; 10(4):412-9. (Clone-specific: Flow cytometry). View Reference
      2. Cantor JM, Ginsberg MH. CD98 at the crossroads of adaptive immunity and cancer. J Cell Sci. 2012; 125(Pt 6):1373-1382. (Biology). View Reference
      3. Ernst DN, McQuitty DN, Weigle WO, Hobbs MV. Expression of membrane activation antigens on murine B lymphocytes stimulated with lipopolysaccharide. Cell Immunol. 1988; 114(1):161-173. (Clone-specific: Flow cytometry). View Reference
      4. Ernst DN, Weigle WO, McQuitty DN, Rothermel AL, Hobbs MV. Stimulation of murine T cell subsets with anti-CD3 antibody. Age-related defects in the expression of early activation molecules. J Immunol. 1989; 142(5):1413-1421. (Clone-specific: Flow cytometry). View Reference
      5. Luscher B, Rousseaux M, Lees R, MacDonald HR, Bron C. Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. II. Structure, biosynthesis, and maturation. J Immunol. 1985; 135(6):3951-3957. (Clone-specific: Immunoprecipitation, Radioimmunoassay). View Reference
      6. MacDonald HR, Lees RK, Bron C. Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. I. Functional characterization by monoclonal antibodies. J Immunol. 1985; 135(6):3944-3950. (Immunogen: (Co)-stimulation, Flow cytometry, Functional assay, Radioimmunoassay). View Reference
      7. Sato Y, Heimeier RA, Li C, Deng C, Shi YB. Extracellular domain of CD98hc is required for early murine development.. Cell Biosci. 2011; 1(1):7. (Biology). View Reference
      View All (7) View Less
      568194 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.