Alexa Fluor™ 647 Mouse Anti-Human MART-1/Melan-A

BD Pharmingen™ Alexa Fluor™ 647 Mouse Anti-Human MART-1/Melan-A

Name: Alexa Fluor™ 647 Mouse Anti-Human MART-1/Melan-A
Brand: BD Pharmingen™
SKU: 567883

Clone A103 (also known as A103)

(RUO)

Flow cytometric analysis of MART-1/Melan-A expression by human SK-MEL-5 cells. Cells from the human SK-MEL-5 (Melanoma, ATCC HTB-70) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The cells were washed and then permeabilized and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat No. 557732, dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human MART-1/Melan-A antibody (Cat No. 567883; solid line histogram). The fluorescence histogram showing MART-1/Melan-A expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact human SK-MEL-5 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

Immunohistofluorescent staining of MART-1/Melan-A expressed in human melanoma tissue. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), sections from formalin-fixed, paraffin-embedded, human melanoma cell-containing tissue were stained with Alexa Fluor™ 647 Mouse Anti-MART-1/Melan-A antibody (Cat. No. 567883; pseudo-colored red) and counterstained with DAPI (Cat. No. 564907, pseudo-colored blue). Original magnification: 20 X.

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Product Details

Brand: BD Pharmingen™

Alternative Name: MART-1; Melan-A; Melan A ; MLANA

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Recombinant Human Melan-A Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)

Concentration: 0.2 mg/ml

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Alexa Fluor™ is a trademark of Life Technologies Corporation.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Fixation Buffer RUO

Size 100 mL Cat No. 554655

Perm/Wash Buffer RUO

Size 100 mL Cat No. 554723

Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO

Size 100 Tests Cat No. 557732

567883 Rev. 1

Antibody Details

A103

The A103 monoclonal antibody specifically recognizes Melanoma antigen recognized by T-cells 1 (MART-1) which is also known as Melan-A. This molecule is an ~13 kDa transmembrane protein that is encoded by MLANA and belongs to a melanocyte lineage-specific family of proteins. MART-1 (Melan-A) is found in melanosomes and the endoplasmic reticulum of melanocytes and melanoma cells as well as retinal pigment epithelium. The A103 antibody labels melanomas and other tumors showing melanocytic differentiation. The A103 antibody can also reportedly stain adrenal cortical cells and steroid-producing cells in the ovary and testis. MART-1 (Melan-A) plays a role in melanosome biosynthesis and can serve as a target antigen recognized by cytotoxic T lymphocytes.

567883 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

567883 Rev.1

Citations & References

Development References (4)

Chen YT, Stockert E, Jungbluth A, et al. Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas.. Proc Natl Acad Sci U S A. 1996; 93(12):5915-9. (Immunogen: Immunohistochemistry, Western blot). View Reference
Coulie PG, Brichard V, Van Pel A, et al. A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas.. J Exp Med. 1994; 180(1):35-42. (Biology). View Reference
Jungbluth AA, Busam KJ, Gerald WL, et al. A103: An anti-melan-a monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues.. Am J Surg Pathol. 1998; 22(5):595-602. (Clone-specific). View Reference
Kawakami Y, Eliyahu S, Delgado CH, et al. Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor.. Proc Natl Acad Sci U S A. 1994; 91(9):3515-9. (Biology). View Reference

View All (4)

567883 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.