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NHP T/B/NK Cell Cocktail

BD Pharmingen™ NHP T/B/NK Cell Cocktail

Product Details
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Product Details

BD Pharmingen™
Rhesus, Cynomolgus, Baboon (QC Testing)
Flow cytometry (Routinely Tested)
RUO
AB_1645290

Description

        Cocktail Component                Clone                Isotype

        FITC anti-Human CD3                SP34-2                mIgG1

        PE anti-Human CD16                3G8                        mIgG1

        APC anti-Human CD20                L27                        mIgG1

The NHP T/B/NK Cocktail is a three-color reagent cocktail designed to identify NHP T, B, and NK lymphocyte populations by direct immunofluorescent staining with flow cytometric analysis.  Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The monoclonal antibody clone L27 reacts with CD20 found only on B lymphocytes.  The monoclonal antibody clone 3G8 reacts with CD16, the 50-65 kDa transmembrane form of IgG Fc (FcγRIII), a human NK-cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
558639 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.