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Purified Hamster IgG2, λ1 Isotype Control
Product Details
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BD Pharmingen™
Anti-KLH
Armenian Hamster IgG2, λ1
Keyhole limpet hemocyanin
ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed, Intracellular staining (flow cytometry), Isotype control (Tested During Development)
250 µg/ml
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

For immunohistochemical staining of acetone-fixed frozen sections, a dilution range of 1:12.5  - 1:50 is suggested.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  5. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550345 Rev. 3
Antibody Details
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Ha4/8

The Ha4/8 antibody is specific for keyhole limpet hemocyanin (KLH). It is useful as an immunoglobulin isotype-matched negative control for immunofluorescent staining.

550345 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550345 Rev.3
Citations & References
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Development References (1)

  1. Mendrick DL, Kelly DM. Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest. 1993; 69(6):690-702. (Immunogen). View Reference
550345 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.