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PE Mouse Anti-Human CD103
Product Details
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ITGAE; Integrin alpha-E; ITAE; HML-1 antigen; Mucosal lymphocyte 1; HUMINAE
Mouse BALB/c IgG1, κ
Human MAPS16 T Cell Line
Flow cytometry
25 μg/mL
20 μL
V A067, BP047, S256
Phosphate buffered saline with gelatin and sodium azide.

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340998 Rev. 1
Antibody Details
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The CD103 antibody, clone Ber-ACT8, is derived using standard cell hybridization procedures using spleen cells from BALB/c mice immunized with the HTLV-1–positive T-cell line, MAPS16.

The CD103 antibody recognizes the αE subunit of integrin αEβ, an integrin also known as the human mucosal lymphocyte (HML) antigen 1, but not α, another member of the βintegrin subfamily. Integrin αEβis a trimeric protein complex of three components, 105 kilodaltons (kDa) (β), 150 kDa, and 25 kDa. The CD103 antibody recognizes an epitope localized on the 150-kDa chain.

340998 Rev. 1
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340998 Rev.1
Citations & References
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Development References (14)

  1. Brew R, West DC, Burthem J, Christmas SE. Expression of the human mucosal lymphocyte antigen, HML-1, by T cells activated with mitogen or specific antigen in vitro. Scan J Immunol. 1995; 41:553–562. (Biology).
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Cerf-Benussan N, Jarry A, Brousse N, Lisowska-Grospierre B, Guy-Grand D, Griscelli C. A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. Eur J Immunol. 1987; 17:1279–1285. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Falini B, Pileri SA, Flenghi L, et al. Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients. Br J Haematol. 1990; 76:460–468. (Biology).
  6. Hadley GA, Rostapshova EA, Gomolka DM, et al. Regulation of the epithelial cell-specific integrin, CD103, by human CD8+ cytolytic T lymphocytes. Transplantation. 1999; 67:1418–1425. (Biology).
  7. Hassan IB, Hagberg H, Sundström C. Immunophenotype of hairy-cell leukemia. Eur J Haematol. 1990; 45:172-176. (Biology).
  8. Kruschwitz M, Fritzsche G, Schwarting R, et al. Ber-ACT8: new monoclonal antibody to the mucosa lymphocyte antigen. J Clin Pathol. 1991; 44(8):636-645. (Biology). View Reference
  9. Lohmeyer J, Friedrish J, Grimminger F, et al. Expression of mucosa-related integrin αEβ7 on alveolar T cells in interstitial lung diseases. Clin Exp Immunol. 1999; 116:340-346. (Biology).
  10. Micklem KJ, Dong Y, Willis A, et al. HML-1 antigen on mucosa-associated T cells, activated cells, and hairy leukemic cells is a new integrin containing the β7 subunit. Am J Pathol. 1996; 3:30-36. (Biology).
  11. Moldenhauer G, Mielke B, Dörken B, Schwartz-Albiez R, Möller P. Identity of HML-1 antigen on intestinal intraepithelial T cells and of B-ly7 antigen on hairy cell leukaemia. Scand J Immunol. 1990; 32:77–82. (Biology).
  12. Möller P, Mielke B, Moldenhauer G. Monoclonal antibody HML-1, a marker for intraepithelial T cells and lymphomas derived thereof, also recognizes hairy cell leukemia and some B-cell lymphomas. Am J Pathol. 1990; 136:509–512. (Biology).
  13. Parker CM, Cepek KL, Russell GJ, et al. A family of beta 7 integrins on human mucosal lymphocytes. Proc Natl Acad Sci U S A. 1992; 89(5):1924-1928. (Biology). View Reference
  14. Rihs S, Walker C, Virchow JC Jr, et al. Differential expression of α E β 7 integrins on bronchoalveolar lavage T lymphocyte subsets: regulation by α 4 β 1-integrin crosslinking and TGF-β. Am J Respir Cell Mol Biol. 1996; 15:600–610. (Biology).
View All (14) View Less
340998 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.