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FITC Mouse Anti-Human CD44
Product Details
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BD™
Pgp-1; H-CAM; Hermes; ECMR-III; HUTCH-1; gp80-95; Ly-24; CSPG8; HCELL
Human
Mouse BALB/c IgG1, κ
Human TCR γδ+ Thymocytes
Flow cytometry
12.5 μg/mL
20 μL
V NK14, S329
960
AB_400360
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347943 Rev. 1
Antibody Details
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L178

The CD44 antibody, clone L178, is derived from hybridization of Sp2/0 myeloma mouse cells with spleen cells from BALB/c mice immunized with TCR-γ/δ+ thymocytes.

The CD44 (Leu-44) antibody recognizes a cell-surface glycoprotein that exists in a variety of isoforms. It is synthesized as a molecule with a molecular weight of 37 kilodaltons (kDa). Upon glycosylation, it is converted to an 80- to 95-kDa form. Alternatively, a 180- to 200-kDa form results from the addition of chondroitin sulfate.

347943 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
347943 Rev.1
Citations & References
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Development References (11)

  1. Aruffo A, Stamenkovic I, Melnick M, Unterhill CB, Seed B. CD44 is the principal cell surface receptor for hyaluronate. Cell. 1990; 61:1303-1313. (Biology).
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Coombe DR, Rider CC. Lymphocyte homing receptors cloned: A role for anionic polysaccharides in lymphocyte adhesion. Immunol Today. 1989; 10:289. (Biology).
  5. Dalchau R. Knapp W, Dörken W, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:622-625.
  6. Gallatin WM, Gale MJ Jr, Hoffman PA, et al. Selective replication of simian immunodeficiency virus in a subset of CD4+ lymphocytes. Proc Natl Acad Sci USA. 1989; 86:3301-3305. (Biology).
  7. Haynes BF, Telen MJ, Hale LP, Denning SM. CD44 – a molecule involved in leukocyte adherence and T-cell activation. Immunol Today. 1989; 10:423-428. (Biology).
  8. Picker LJ, de los Toyos J, Telen MJ, Haynes BF, Butcher EC. Monoclonal antibodies against the CD44 (In-[Lu]-related p80) and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors. J Immunol. 1989; 142:2046-2051. (Biology).
  9. Shimizu Y, Van Seventer GA, Siraganian R, Wahl L, Shaw S. Dual role of the CD44 molecule in T-cell adhesion and activation. J Immunol. 1989; 143:2457-2463. (Biology).
  10. St John T, Meyer J, Idzerda R, Gallatin WM. Expression of CD44 confers a new adhesive phenotype on transfected cells. Cell. 1990; 60:45-52. (Biology).
  11. Stoll M, Dalchau R, Schmidt RE. Knapp W, Dörken W, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:619-622.
View All (11) View Less
347943 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.