Preparation And Storage
The monoclonal antibody is supplied as 12.5 µg purified immunoglobulin in 2.0 mL (6.25 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 12.5 µg in 2.0 mL (6.25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
CD18 (Anti-LFA-1β), clone L130, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with thymic T-cell antigen receptor (TCR)-γ/δ+ T lymphocytes. CD18 (Anti-LFA-1β) recognizes the Mr 95-kdalton (kDa) β chain of the leucocyte function–associated antigen-1 (LFA-1). LFA-1, a member of a subfamily of integrin receptors, is a leucocyte adhesion receptor that binds to an intercellular adhesion molecule (ICAM) on target tissues. This subfamily consists of three heterodimeric proteins that share a common noncovalently-associated β chain (CD18 antigen) but possess distinct α-subunits recognized by CD11a (anti-LFA-1α), CD11b (Leu-15), and CD11c (Leu-M5).
Development References (7)
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Clayberger C, Wright A, Medeiros LJ, et al. Absence of cell surface LFA-1 as a mechanism of escape from immunosurveillance.. Lancet. 1987; 2(8558):533-6. (Biology). View Reference
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Dustin ML, Springer TA. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.. Nature. 1989; 341(6243):619-24. (Biology). View Reference
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Fischer A, Griscelli C, Blanche S, et al. Prevention of graft failure by an anti-HLFA-1 monoclonal antibody in HLA-mismatched bonemarrow transplantation. Lancet. 1986; 1058-1061. (Biology).
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Inghirami G, Wieczorek R, Zhu BY, Silber R, Dalla-Favera R, Knowles DM. Differential expression of LFA-1 molecules in non-Hodgkin's lymphoma and lymphoid leukemia. Blood. 1988; 72(4):1431-1434. (Biology). View Reference
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Krensky AM, Sanchez-Madrid F, Robbins E, Nagy JA, Springer TA, Burakoff SJ. The functional significance, distribution, and structure of LFA-1, LFA-2, and LFA-3: cell surface antigens associated with CTL-target interactions.. J Immunol. 1983; 131(2):611-6. (Biology). View Reference
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Mentzer SJ, Faller DV, Burakoff SJ. Interferon-gamma induction of LFA-1-mediated homotypic adhesion of human monocytes.. J Immunol. 1986; 137(1):108-13. (Biology). View Reference
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Uciechowski P, Schmidt RE. Knapp W, Dörken W, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:543.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.