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      APC Mouse Anti-Human IL-2
      Product Details
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      BD FastImmune™
      IL2; Interleukin-2; T-cell growth factor; TCGF
      Human
      Mouse BALB/c IgG1, κ
      Human IL-2 protein
      Intracellular staining (flow cytometry)
      6 μg/mL
      5 μL
      3558
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO


      Preparation And Storage

      The FITC conjugate is supplied as 12.5 µg in 1.0 mL (12.5 µg/mL) of PBS. The PE conjugate is supplied as 1.6 µg in 1.0 mL (1.6 µg/mL) of PBS. The APC conjugate is supplied as 3 µg in 0.5 mL (6 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      341116 Rev. 1
      Antibody Details
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      5344.111

      Anti-Hu–IL-2, clone 5344.111, is derived from a fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-2.

      Anti-Human Interleukin-2 (Anti-Hu–IL-2) recognizes a 15- to 18-kilodalton (kd) glycoprotein.

      341116 Rev. 1
      Format Details
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      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      341116 Rev.1
      Citations & References
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      Development References (23)

      1. Aggarwal B, Puri R. Human Cytokines: Their Role in Disease and Therapy. Cambridge, MA: Blackwell Science; 1995:3-24.
      2. Clerici M, Hakim FT, Venzon DJ, et al. Changes in interleukin-2 and interleukin-4 production in asymptomatic, human immunodeficiency virus-seropositive individuals. J Clin Invest. 1993; 91:759-765. (Biology).
      3. Clerici M, Shearer GM. A TH1 → TH2 switch is a critical step in the etiology of HIV infection. Today. 1993; 14:107-110. (Biology).
      4. Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
      5. Croft M, Carter L, Swain S, Dutton RW. Generation of polarized antigen-specific CD8 effector populations: reciprocal action of interleukin (IL)-4 and IL-12 in promoting Type 2 versus Type 1 cytokine profiles. J Exp Med. 1994; 180:1715-1728. (Biology).
      6. ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology).
      7. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+ CD27- lymphocyte subpopulation. J Immunol. 1995; 154:4294-4301. (Biology).
      8. Graves JD, Downward J, Izquierdo-Pastor M, Rayter S, Warne PH, Cantrell DA. The growth factor IL-2 activates p21ras proteins in normal human lymphocytes. J Immunol. 1992; 148:2417-2422. (Biology).
      9. Graziosi C, Pantaleo G, Gantt KR, et al. Lack of evidence for the dichotomy of T H 1 and T H 2 predominance in HIV-infected individuals. Science. 1994; 265:248-252. (Biology).
      10. Gusella GL, Musso T, Bosco MC, Expinoza-Delgado I, Matsushima K, Varesio L. IL-2 upregulates but IFN-γ suppresses IL-8 expression in human monocytes. J Immunol. 1993; 151:2725-2732. (Biology).
      11. Hillman GG, Haas GP. Role of cytokines in lymphocyte functions. Human Cytokines: Their Role in Disease and Therapy. 1995; 37-54. (Biology).
      12. Hyjek E, Lischner HW, Hyslop T, et al. Cytokine patterns during progression to AIDS in children with perinatal HIV infection. J Immunol. 1995; 155:4060-4071. (Biology).
      13. Lanier LL, Benike CJ, Phillips JH, Engleman EG. Recombinant interleukin-2 enhanced natural killer cell– mediated cytotoxicity in human lymphocyte subpopulations expressing the Leu-7 and Leu-11 antigens. J Immunol. 1985; 134:794-801. (Biology).
      14. Maggi E, Mazzetti M, Ravina A, et al. Ability of HIV to promote a TH1 to TH0 shift and to replicate preferentially in TH2 and TH 0 cells. Science. 1994; 265:244-248. (Biology).
      15. Musso T, Espinoza-Dalgado I, Pullki K, Gusella G, Longo D, Varesio L. IL-2 induces IL-6 production in human monocytes. J Immunol. 1992; 148:795-800. (Biology).
      16. Openshaw P, Murphy EE, Hosken NA, et al. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med. 1995; 182(5):1357-1367. (Biology). View Reference
      17. Phillips JH, Gemlo BT, Myers WW, Rayner AA, Lanier LL. In vivo and in vitro activation of natural killer cells in advanced cancer patients undergoing combined recombinant interleukin-2 and LAK cell therapy. J Clin Oncology. 1987; 5:1933-1941. (Biology).
      18. Picker LJ, Singh MK, Zdraveski Z, et al. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry. Blood. 1995; 86:1408-1419. (Biology).
      19. Röcken M, Müller KM, Saurat J-H, et al. Central role for TCR/CD3 ligation in the differentiation of CD4+ T cells toward a TH1 or TH2 functional phenotype. J Immunol. 1992; 148:1031-1036. (Biology).
      20. Röcken M, Saurat J-H, Hauser C. A common precursor for CD4+ T cells producing IL-2 or IL-4. J Immunol. 1992; 148:47-54. (Biology).
      21. Street NE, Mosmann TR. Functional diversity of T lymphocytes due to secretion of different cytokine patterns. FASEB J. 1991; 5:171-176. (Biology).
      22. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.. J Immunol Methods. 1998; 212(1):89-98. (Biology). View Reference
      23. Zhang X, Giangreco L, Broome E, Dargan CM, Swain SL. Control of CD4 effector fate: transforming growth factor β 1 and interleukin-2 synergize to prevent apoptosis and promote effector expansion. J Exp Med. 1995; 182:669-709. (Biology).
      View All (23) View Less
      341116 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

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